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Citation
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HERO ID
3350269
Reference Type
Journal Article
Title
Quantitative proteomic analyses of mammary organoids reveals distinct signatures after exposure to environmental chemicals
Author(s)
Williams, KE; Lemieux, GA; Hassis, ME; Olshen, AB; Fisher, SJ; Werb, Z
Year
2016
Is Peer Reviewed?
1
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN:
0027-8424
EISSN:
1091-6490
Book Title
Proc Natl Acad Sci U S A.
Volume
113
Issue
10
Page Numbers
E1343-E1351
Language
English
PMID
26903627
DOI
10.1073/pnas.1600645113
Web of Science Id
WOS:000372013300006
URL
https://search.proquest.com/docview/1774332982?accountid=171501
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Abstract
Common environmental contaminants such as bisphenols and phthalates and persistent contaminants such as polychlorinated biphenyls are thought to influence tissue homeostasis and carcinogenesis by acting as disrupters of endocrine function. In this study we investigated the direct effects of exposure to bisphenol A (BPA), mono-n-butyl phthalate (Pht), and polychlorinated biphenyl 153 (PCB153) on the proteome of primary organotypic cultures of the mouse mammary gland. At low-nanomolar doses each of these agents induced distinct effects on the proteomes of these cultures. Although BPA treatment produced effects that were similar to those induced by estradiol, there were some notable differences, including a reduction in the abundance of retinoblastoma-associated protein and increases in the Rho GTPases Ras-related C3 botulinum toxin substrate 1 (Rac1) and cell division cycle protein CDC42. Both Pht and PCB153 induced changes that were distinct from those induced by estrogen, including decreased levels of the transcriptional corepressor C-terminal binding protein 1. Interestingly, the three chemicals appeared to alter the abundance of distinct splice forms of many proteins as well as the abundance of several proteins that regulate RNA splicing. Our combined results indicate that the three classes of chemical have distinct effects on the proteome of normal mouse mammary cultures, some estrogen-like but most estrogen independent, that influence diverse biological processes including apoptosis, cell adhesion, and proliferation.
Keywords
proteomics; mammary epithelium; environmental chemicals; organotypic culture; estrogen
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IRIS
•
Dibutyl Phthalate (DBP)
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Litsearch Jan 2016 - July 2016
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LitSearch Jul 2016 - Jan 2017
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PCBs
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Litsearch Aug 2015 - Aug 2016
Pubmed
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Phthalates – Targeted Search for Epidemiological Studies
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Source - Dec 2016 Update (Private)
Pubmed
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