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HERO ID
3350340
Reference Type
Journal Article
Title
SPE-UPLC-MS/MS for the determination of phthalate monoesters in rats urine and its application to study the effects of food emulsifier on the bioavailability of priority controlling PAEs
Author(s)
Xu, R; Gao, HT; Zhu, F; Cao, WX; Yan, YH; Zhou, X; Xu, Q; Ji, WL
Year
2016
Is Peer Reviewed?
Yes
Journal
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ISSN:
1570-0232
EISSN:
1873-376X
Publisher
Elsevier B.V.
Volume
1012-1013
Page Numbers
97-105
Language
English
PMID
26815919
DOI
10.1016/j.jchromb.2016.01.007
Web of Science Id
WOS:000370882800013
URL
http://www.sciencedirect.com/science/article/pii/S1570023216300071
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Abstract
This research was mainly focused on the effects of food emulsifier on the bioavailability of six priority controlling phthalate acid esters (PAEs) for the further accurate assessment of their toxic effects, using the corresponding phthalate acid monoesters (PAMEs) in rats urine as biomarkers. Glycerin monostearate was chosen as typical food emulsifier. A method was established to determine PAMEs in urine from rats either in experimental group (integrated gavaged with glycerin monostearate and PAEs) or in control group (gavaged with PAEs only), by using solid-phase extraction (SPE) coupled with ultra performance liquid chromatography tandem mass spectrometry (SPE-UPLC-MS/MS). Extraction recoveries were more than 75% for all the PAMEs; the calibration curve was linear in the range of 1.0-1000.0ng/mL with R(2)>0.995; the limits of detection (LOD) were 0.30ng/mL-0.50ng/mL. In addition, by analysing quality control (QC) urine samples in 3 days, it showed that the method was precise and accurate, for the intra-day and inter-day RSD within 16%, and the accuracy more than 82%. Internal exposure amount of all PAEs in experimental group was significantly higher than that in control group with p values of less than 0.05 except for butyl benzyl phthalates (BBP) (P=0.07). The bioavailability of all PAEs ranged from 5.03% to 109.35% with the presence of food emulsifiers glycerin monostearate, observably higher than that without glycerin monostearate (1.12% to 54.39%). It indicated that food emulsifiers increased the bioavailability of PAEs and may lead to potential food safety risk, which should bring awareness and be further studied.
Keywords
Phthalic acid esters; Glycerin monostearate; Bioavailability; Phthalate acid monoesters; Solid-phase extraction; UPLC-MS/MS
Tags
IRIS
•
BBP (Butyl benzyl phthalate)
Literature Search
Literature Search: January 2016 - June 2016
Pubmed
WOS
Literature Search: July 2016 - January 2017
WOS
Excluded: No Primary Data on Health Effects
Measurement methods
•
Dibutyl Phthalate (DBP)
Database Searches
Litsearch Jan 2016 - July 2016
Pubmed
Excluded: No Primary Data on Health Effects
Measurement methods
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