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3350550 
Journal Article 
Development of an immunochromatographic test strip and ic-ELISA for tetrabromobisphenol: a detection in lake water and rice pudding samples 
Yu, Li; Liu, L; Song, S; Kuang, Hua; Xu, C 
2016 
Yes 
Food and Agricultural Immunology
ISSN: 0954-0105
EISSN: 1465-3443 
Food and Agricultural Immunology 
27 
4 
460-470 
WOS:000374693800003 
Abstract: A sensitive and selective indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic test strip were developed for the detection of tetrabromobisphenol A (TBBPA), an important brominated flame retardant. A monoclonal antibody (mAb) against TBBPA was produced and the half inhibition concentration (IC50) was 0.44ng/ml, with the linear range 0.15-1.32ng/ml. TBBPA recovery by ic-ELISA was 70.7-108.8% from rice pudding samples and 110.2-119.8% from lake water samples. A sensitive and rapid immunochromatographic test strip was developed using this specific mAb, with a good cut-off value of 25 and 50ng/ml in lake water and rice pudding samples, respectively. The results of this study revealed that the ic-ELISA and immunochromatographic test strip represent useful methods of TBBPA detection in lake water and rice pudding samples. ds: Food Science & Technology cument Delivery No.: DK1SH 
Tetrabromobisphenol A; ELISA; immunochromatographic test strip; lake water; rice pudding