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Citation
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HERO ID
3350726
Reference Type
Journal Article
Title
Photoconversion and Fluorescence Properties of a Red/Green-Type Cyanobacteriochrome AM1_C0023g2 That Binds Not Only Phycocyanobilin But Also Biliverdin
Author(s)
Fushimi, K; Nakajima, T; Aono, Y; Yamamoto, T; Ni-Ni-Win, T; Ikeuchi, M; Sato, M; Narikawa, R
Year
2016
Is Peer Reviewed?
Yes
Journal
Frontiers in Microbiology
ISSN:
1664-302X
Volume
7
Page Numbers
588
Language
English
PMID
27242674
DOI
10.3389/fmicb.2016.00588
Abstract
Cyanobacteriochromes (CBCRs) are distantly related to the red/far-red responsive phytochromes. Red/green-type CBCRs are widely distributed among various cyanobacteria. The red/green-type CBCRs covalently bind phycocyanobilin (PCB) and show red/green reversible photoconversion. Recent studies revealed that some red/green-type CBCRs from chlorophyll d-bearing cyanobacterium Acaryochloris marina covalently bind not only PCB but also biliverdin (BV). The BV-binding CBCRs show far-red/orange reversible photoconversion. Here, we identified another CBCR (AM1_C0023g2) from A. marina that also covalently binds not only PCB but also BV with high binding efficiencies, although BV chromophore is unstable in the presence of urea. Replacement of Ser334 with Gly resulted in significant improvement in the yield of the BV-binding holoprotein, thereby ensuring that the mutant protein is a fine platform for future development of optogenetic switches. We also succeeded in detecting near-infrared fluorescence from mammalian cells harboring PCB-binding AM1_C0023g2 whose fluorescence quantum yield is 3.0%. Here the PCB-binding holoprotein is shown as a platform for future development of fluorescent probes.
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IRIS
•
PCBs
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Litsearches
Remaining
Litsearch Aug 2015 - Aug 2016
Pubmed
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