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Citation
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HERO ID
3453271
Reference Type
Journal Article
Title
Impaired self-healing capacity in airway epithelia lacking aquaporin-3
Author(s)
Zhu, HX; Zhou, JB; Zhu, XD; Zhou, J; Li, J; Song, YL; Bai, CX
Year
2016
Is Peer Reviewed?
1
Journal
Respiratory Physiology and Neurobiology
ISSN:
1569-9048
EISSN:
1878-1519
Volume
233
Page Numbers
66-72
Language
English
PMID
27524635
DOI
10.1016/j.resp.2016.08.002
Web of Science Id
WOS:000386410600010
URL
http://
://WOS:000386410600010
Exit
Abstract
In this study, we utilized AQP3-knockout mice as the in vivo model and AQP3-knockdown human bronchial epithelial cells (HBECs) as the in vitro model. Airway injury was experimentally induced by intra-tracheal injection of naphthalene. HE staining, transmission and scanning electron microscope were performed to evaluate self-healing capacity in vivo. Transwell and wound-healing assays were performed to evaluate epithelial cell migration in vitro. We found that both the airway epithelial cells of AQP3-knockout mice and AQP3-knockdown HBECs exhibited an obviously impaired self-healing capacity with defective epithelial cell migration through AQP3-facilitated glycerol transport. In addition, glycerol supplementation could largely correct defective injury healing and epithelial cell migration. For the first time, we found evidence for distinct defects in AQP3-deficient airway epithelial cell migration. Mechanistic analysis showed AQP3-facillitated glycerol transport plays a role in airway epithelial self-healing after injury.
Tags
IRIS
•
Naphthalene
Database searches - Jan 2017 (private)
New Jan 2017 (private)
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PubMed
WOS
Combined data set
Data set for title/abstract screening
Data set for full text review
Excluded – PECO criteria not met (full-text)
Supplemental material
Non-PECO routes of exposure
Other
•
Naphthalene (2021 Evidence mapping publication)
Database searches - Jan 2017 (private)
New Jan 2017 (private)
Database Searches
PubMed
WOS
Combined data set
Data set for title/abstract screening
Data set for full text review
Excluded – PECO criteria not met (full-text)
Supplemental material
Exposure routes other than inhalation, oral, or dermal (animal studies)
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