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HERO ID
3487216
Reference Type
Journal Article
Title
Chloroform-induced oxidative stress in rat liver: Implication of metallothionein
Author(s)
Abbassi, R; Chamkhia, N; Sakly, M
Year
2010
Is Peer Reviewed?
1
Journal
Toxicology and Industrial Health
ISSN:
0748-2337
EISSN:
1477-0393
Volume
26
Issue
8
Page Numbers
487-496
Language
English
PMID
20529963
DOI
10.1177/0748233710373088
Web of Science Id
WOS:000281383400005
Abstract
Current studies evaluated the effect of acute and subacute exposure to chloroform (CHCl(3)) on rat liver and the implication of oxidative stress. For this purpose, different doses of CHCl(3) (150, 300 and 450 mg/kg bw) were administered intraperitoneally (ip) to male Wistar rats. Malondialdehyde (MDA), glutathione (GSH), reduced cytochrome c and metallothioneins (MTs) levels as well as the activities of catalase (CAT) and glutathione peroxidase (GPx) and the activities of the biochemical markers of hepatic injury (alanine transaminase [ALT] and aspartate transaminase [AST]) were determined. CHCl(3) did not cause a significant increase in hepatic lipid peroxidation. However, dose-dependant and/or time dependant effects of CHCl(3) were demonstrated on most of the oxidative stress parameters measured, namely the GSH depletion and the superoxide anion production. Acute exposure CHCl(3) increased the aminotransferase and GPx activities and reduced cytochrome c levels in a dose-dependant pattern. A well-combined dose-dependent and time-dependent effect of CHCl(3) on MT levels after acute and subacute exposure was noticed. Moreover, the increase of MT levels seems to be associated with the GSH depletion, indicating a possible role of the latter in MT synthesis. In conclusion, the superoxide anion production and the GSH depletion could be implicated in the mechanism of hepatotoxity of CHCl(3) and MTs seem to be a part of the antioxidant defense system against the oxidative damage caused by CHCl(3) in liver.
Tags
•
Chloroform 2018 Update
TITLE AND ABSTRACT REVIEW
Included Studies
FULL-TEXT REVIEW
Excluded
Excluded, but considered supplemental information
•
Chloroform Combined (current)
Chloroform (original)
References: 2000-2018
WoS
PubMed
Chloroform (2018 update)
TITLE AND ABSTRACT REVIEW
Included Studies
FULL-TEXT REVIEW
Excluded
Excluded, but considered supplemental information
Chloroform (current)
Literature Search: Jan 2009 - March 2017
PubMed
OPPT REs
•
OPPT_Carbon Tetrachloride_C. Engineering
Total – title/abstract screening
Off topic
•
OPPT_Carbon Tetrachloride_D. Exposure
Total – title/abstract screening
Off topic
•
OPPT_Carbon Tetrachloride_E. Fate
Total – title/abstract screening
Off topic
•
OPPT_Carbon Tetrachloride_F. Human Health
Total – title/abstract screening
Off topic
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