Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
3515165
Reference Type
Journal Article
Title
Carboxylesterase-involved metabolism of di-n-butyl phthalate in pumpkin (Cucurbita moschata) seedlings
Author(s)
Lin, Q; Chen, S; Chao, Y; Huang, X; Wang, S; Qiu, R
Year
2017
Is Peer Reviewed?
Yes
Journal
Environmental Pollution
ISSN:
0269-7491
EISSN:
1873-6424
Publisher
ELSEVIER SCI LTD
Location
OXFORD
Volume
220
Issue
Pt A
Page Numbers
421-430
Language
English
PMID
27697378
DOI
10.1016/j.envpol.2016.09.084
Web of Science Id
WOS:000390736700046
URL
https://search.proquest.com/docview/2116899505?accountid=171501
Exit
Abstract
Uptake and accumulation by plants is a significant pathway in the migration and transformation of phthalate esters (PAEs) in the environment. However, limited information is available on the mechanisms of PAE metabolism in plants. Here, we investigated the metabolism of di-n-butyl phthalate (DnBP), one of the most frequently detected PAEs, in pumpkin (Cucurbita moschata) seedlings via a series of hydroponic experiments with an initial concentration of 10 mg L(-1). DnBP hydrolysis occurred primarily in the root, and two of its metabolites, mono-n-butyl phthalate (MnBP) and phthalic acid (PA), were detected in all plant tissues. The MnBP concentration was an order of magnitude higher than that of PA in shoots, which indicated MnBP was more readily transported to the shoot than was PA because of the former's dual hydrophilic and lipophilic characteristics. More than 80% of MnBP and PA were located in the cell water-soluble component except that 96% of MnBP was distributed into the two solid cellular fractions (i.e., cell wall and organelles) at 96 h. A 13-20% and 29-54% increase of carboxylesterase (CXE) activity shown in time-dependent and concentration-dependent experiments, respectively, indicated the involvement of CXEs in plant metabolism of DnBP. The level of CXE activity in root subcellular fractions was in the order: the cell water-soluble component (88-94%) > cell wall (3-7%) > cell organelles (3-4%), suggesting that the cell water-soluble component is the dominant locus of CXE activity and also the domain of CXE-catalyzed hydrolysis of DnBP. The addition of triphenyl phosphate, a CXE inhibitor, led to 43-56% inhibition of CXE activity and 16-25% increase of DnBP content, which demonstrated the involvement of CXEs in plant metabolism of DnBP. This study contributes to our understanding of enzymitic mechanisms of PAE transformation in plants.
Keywords
Di-n-butyl phthalate (DnBP); Carboxylesterases; Metabolism; Pumpkin (Cucurbita moschata); Subcellular distribution
Tags
IRIS
•
Dibutyl Phthalate (DBP)
Database Searches
LitSearch Jul 2016 - Jan 2017
Considered new
PubMed
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity