US EPA

3539328 

Journal Article 

Enzymatic synthesis of ascorbyl ester derived from linoleic acid 

Balen, M; Gomes, GR; Kratz, JM; Simões, CM; Valério, A; de Oliveira, D 

2017 

Yes 

Bioprocess and Biosystems Engineering
ISSN: 1615-7591
EISSN: 1615-7605 

40 

2 

265-270 

English 

27752771 

10.1007/s00449-016-1694-6 

Antioxidants are substances that defend cells against damage, kidnapping and destroying free radicals. They have been largely used in the food industry due the possibility to control the oxidation process, aimed to increase shelf life. Thus, esterification reaction to obtain ascorbyl linoleate catalyzed by Novozym 435 lipase assisted by ultrasound bath was investigated. In this work, molecular sieve (4 Å) was added to the reaction medium to remove the water formed during the esterification reaction to improve the process performance. According to the results, ascorbyl linoleate production up to 90 % was reached after 1 h of reaction time carried out using ultrasound bath, 1:9 molar ratio of substrates L-ascorbic acid to linoleic acid, 20 mL of tert-butanol as organic solvent, 5 wt% of Novozym 435 lipase, 10 wt% of molecular sieve at 70 °C.