US EPA

3540857 

Journal Article 

How to solve the problem of co-elution between two compounds in liquid chromatography through the first UV derivative spectrum. A trial on alternative plasticizers to di(2-ethylhexyl) phthalate 

Masse, M; Genay, S; Feutry, F; Simon, N; Barthélémy, C; Sautou, V; Décaudin, B; Odou, P 

2017 

1 

Talanta
ISSN: 0039-9140
EISSN: 1873-3573 

ELSEVIER 

AMSTERDAM 

162 

187-192 

English 

27837816 

10.1016/j.talanta.2016.10.029 

WOS:000389088700026 

https://linkinghub.elsevier.com/retrieve/pii/S0039914016307664
Exit
 

To meet new regulations, alternative plasticizers to di(2-ethylhexyl) phthalate (DEHP) are now commonly used in the manufacturing of medical devices. These are: acetyl tri-n-butyl citrate (ATBC), bis (2-ethylhexyl)adipate (DEHA), dioctyl terephtalate (DEHT), di-isononylphtalate (DINP), diisononylcyclohexane-1.2-dicarboxylate (DINCH) and trioctyltrimellilate (TOTM). An HPLC-UV analysis was previously developed to characterize four of them. However, two compounds were systematically co-eluated: DEHP with DEHA and DEHT with DINP. The first derivative of UV spectra and photodiode array detection allow the quantification of DEHA and DINP. Moreover, for each plasticizer, maximum wavelength absorbance was chosen to be as specific as possible. Quantification ranged from 0.3 to 750µg/mL according to the plasticizer. The assays were validated by analysis of variance. Our method was validated by determining the following parameters: specificity, linearity, limits of detection and quantification. The relative biases were inferior to 5% for ATBC, DEHP, DEHA and DINCH and inferior to 10% for DEHT, DINP and TOTM. Plasticizers were extracted with tetrahydrofuran and methanol. The developed method was then used to determine the composition of plasticizers in several medical devices used in clinical service. The major plasticizers were quantified from 19% to 40% w/w, traces of DEHT were found in six medical devices and DEHP in five. 

chromatography; high pressure liquid/methods; plasticizers/analysis; diethylhexyl phthalate/analysis; materials testing; polyvinyl chloride/analysis