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HERO ID
3719812
Reference Type
Journal Article
Title
Live and heat-killed Lactobacillus spp. interfere with Streptococcus mutans and Streptococcus oralis during biofilm development on titanium surface
Author(s)
Ciandrini, E; Campana, R; Baffone, W
Year
2017
Is Peer Reviewed?
Yes
Journal
Archives of Oral Biology
ISSN:
0003-9969
Volume
78
Page Numbers
48-57
Language
English
PMID
28193570
DOI
10.1016/j.archoralbio.2017.02.004
Abstract
OBJECTIVES:
This research investigates the ability of live and heat-killed (HK) Lactic Acid Bacteria (LAB) to interfere with Streptococcus mutans ATCC 25175 and Streptococcus oralis ATCC 9811 during biofilm formation.
DESIGN:
Eight Lactobacillus spp. and two oral colonizers, pathogenic Streptococcus mutans and resident Streptococcus oralis, were characterized for their aggregation abilities, cell surface properties and biofilm formation ability on titanium surface. Then, the interference activity of selected live and HK Lactobacillus spp. during S. mutans and S. oralis biofilm development were performed. The cell-free culture supernatants (CFCS) anti-biofilm activity was also determined.
RESULTS:
LAB possess good abilities of auto-aggregation (from 14.19 to 28.97%) and of co-aggregation with S. oralis. The cell-surfaces characteristics were most pronounced in S. mutans and S. oralis, while the highest affinities to xylene and chloroform were observed in Lactobacillus rhamnosus ATCC 53103 (56.37%) and Lactobacillus paracasei B21060 (43.83%). S. mutans and S. oralis developed a biofilm on titanium surface, while LAB showed a limited or no ability to create biofilm. Live and HK L. rhamnosus ATCC 53103 and L. paracasei B21060 inhibited streptococci biofilm formation by competition and displacement mechanisms with no substantial differences. The CFCSs of both LAB strains, particularly the undiluted one of L. paracasei B21060, decreased S. mutans and S. oralis biofilm formation.
CONCLUSIONS:
This study evidenced the association of LAB aggregation abilities and cell-surface properties with the LAB-mediated inhibition of S. mutans and S. oralis biofilm formation. Lactobacilli showed different mechanisms of action and peculiar strain-specific characteristics, maintained also in the heat-killed LAB.
Tags
IRIS
•
Chloroform 2018 Update
TITLE AND ABSTRACT REVIEW
Excluded Studies
•
Chloroform Combined (current)
Chloroform (2018 update)
TITLE AND ABSTRACT REVIEW
Excluded Studies
Chloroform (current)
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