Profiling of urinary medium-sized peptides in normal and uremic urine by high-performance liquid chromatography | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

3858401

Reference Type

Journal Article

Title

Profiling of urinary medium-sized peptides in normal and uremic urine by high-performance liquid chromatography

Author(s)

Mabuchi, H; Nakahashi, H

Year

1982

Is Peer Reviewed?

Journal

Journal of Chromatography
ISSN: 0021-9673

Volume

233

Issue

Page Numbers

107-113

Language

English

PMID

7161326

DOI

10.1016/s0378-4347(00)81736-3

Abstract

This report describes the profiling of medium-sized peptides in both normal and uremic urine by ion-pair reversed-phase high-performance liquid chromatography using an acetonitrile--heptafluorobutyric acid solvent system as eluent. Several medium-sized peptide peaks could be detected in both normal and uremic urine at low picomole level by using post-column fluorescence derivatization with fluorescamine. Contrary to expectation, uremic urine contained slightly larger amounts of medium-sized peptides compared with normal urine.