US EPA

3858440 

Journal Article 

VOLATILE ION-PAIRING AGENTS FOR LIQUID-CHROMATOGRAPHIC THERMOSPRAY MASS-SPECTROMETRIC DETERMINATION OF AMINO-ACIDS AND AMINO-ACID AMIDES 

Vanleuken, RGJ; Kwakkenbos, GTC; Duchateau, ALL 

1993 

Yes 

Journal of Chromatography A
ISSN: 0021-9673
EISSN: 1873-3778 

Elsevier 

647 

1 

131-136 

English 

10.1016/0021-9673(93)83332-M 

WOS:A1993LX50900016 

http://linkinghub.elsevier.com/retrieve/pii/002196739383332M
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The behaviour of volatile ion-pairing agents in terms of performance of the combined liquid chromatography-mass spectrometry is described. The applicability of heptafluorobutanoic acid, tridecafluoroheptanoic acid and nonadecafluorodecanoic acid as volatile ion-pairing agents is demonstrated by the analysis of mixtures of amino acids and the corresponding amino acid amides. Translation of a non-volatile to a volatile ion-pair system that is suitable for thermospray mass spectrometry is described. Using nonadecafluorodecanoic acid, the retention times of the compounds studied were comparable to those obtained with n-dodecylsulphonic acid. Further, with nonadecafluorodecanoic acid, the repeatability of the tests and the calibration graphs of the compounds investigated were good. The sensitivity of the mass spectrometer towards the compounds of interest was greatly improved by postcolumn addition of trifluoroacetic acid and ionization with gaseous ammonia. Using nonadecafluorodecanoic acid, the method was applied to the analysis of samples from bio-organic synthesis.