US EPA

3858731 

Journal Article 

Quantitative determination of pentazocine in plasma and of pentazocine and metabolites in urine 

Pittman, KA; Davison, C 

1973 

Yes 

Journal of Pharmaceutical Sciences
ISSN: 0022-3549
EISSN: 1520-6017 

IPA/74/110132 

Pharm 

REF 15 

765-769 

English 

10.1002/jps.2600620512 

WOS:A1973P596200011 

IPA COPYRIGHT: ASHP A gas chromatographic method for the determination of pentazocine and its metabolites in human plasma and urine is described. The determination of plasma pentazocine involves the extraction of pentazocine and a suitable internal standard, the derivatization of these compounds with heptafluorobutyric acid anhydride, and the chromatography of the derivatives using tritium foil, electron capture detection. The determination of urinary pentazocine and the trans-alcohol and trans-acid metabolite involved the extraction of the compounds and an internal standard, derivatization with a silylating reagent, and the chromatography of the derivatives using flame-ionization detection. The determination of the cis-alcohol metabolite involved adsorption chromatography followed by the same derivatization and chromatographic analysis as used for the trans derivatives. The lower limit for the quantitative procedure described was found to be about 0.1-0.2 mcg./ml. for pentazocine and its hydroxylated metabolites, and about 1-2 mcg./ml. for the acid metabolites. 

GLC—analysis, pentazocine in plasma, pentazocine and metabolites in urine; Pentazocine in plasma, pentazocine and metabolites in urine—GLC analysis