Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
3859026
Reference Type
Journal Article
Title
Development of a homologous fluorescence polarization immunoassay for diisobutyl phthalate in romaine lettuce
Author(s)
Chen, Y; Cui, X; Wu, P; Jiang, Z; Jiao, L; Hu, Q; Eremin, SA; Zhao, S
Year
2017
Is Peer Reviewed?
Yes
Journal
Food Analytical Methods
ISSN:
1936-9751
Publisher
Springer Nature B.V.
Location
New York
Volume
10
Issue
2
Page Numbers
449-458
Language
English
DOI
10.1007/s12161-016-0596-1
Web of Science Id
WOS:000392379600019
URL
https://search.proquest.com/docview/2000507081?accountid=171501
Exit
Abstract
In this work, three fluorescein-labeled DiBP derivatives (tracers) with different chemical structures and spacer bridges were synthesized and purified by thin-layer chromatography (TLC). Compared with the heterologous tracer, the homologous tracer exhibited more affinity to the antibody. What is more, the tracer concentration and the antibody dilution were further evaluated to improve the sensitivity of fluorescence polarization immunoassay (FPIA). On the basis of sensitive antibody and tracer, a rapid and specific FPIA has been established for the detection of DiBP contamination in the romaine lettuce, which has rarely been reported before. Under the optimal conditions, the developed FPIA showed a good detection range from 8.82 to 2152.84 ng/mL, with a detection limit of 1.77 ng/mL. In addition, the cross-reactivity to several compounds structurally related to DiBP was less than 7.37 %. Therefore, DiBP contamination in spiked romaine lettuce samples was detected by FPIA, with the recovery from 88.28 to 119.11 %. Moreover, when compared with high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA), the results analyzed by the developed FPIA shows a strong reliability and a high correlation value of 0.978 and 0.980, respectively. Thus, this data, combined with rapidity and simplicity of the assay, demonstrates that the established FPIA is a suitable method for high throughput screening of DiBP contamination in the romaine lettuce.
Keywords
Fluorescence polarization immunoassay; Phthalate acid esters; Diisobutyl phthalate; Romaine lettuce
Tags
IRIS
•
Diisobutyl Phthalate (DIBP) Final
Database Searches
January 2017 Update
July 2017 Update
Web of Science
New for this search
No Primary Data on Toxic Effects
Measurement methods
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity