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HERO ID
3859124
Reference Type
Journal Article
Title
Three generation study of reproductive and developmental toxicity following exposure of pubescent F0 male mice to di-n-butyl phthalate
Author(s)
Dobrzynska, MM; Tyrkiel, EJ; Gajowik, A
Year
2017
Is Peer Reviewed?
1
Journal
Mutagenesis
ISSN:
0267-8357
EISSN:
1464-3804
Book Title
Mutagenesis.
Volume
32
Issue
4
Page Numbers
445-454
Language
English
PMID
28472404
DOI
10.1093/mutage/gex011
Web of Science Id
WOS:000405662100005
Abstract
Humans are exposed to phthalates continuously throughout life. The aim of this study was to evaluate the genotoxic effects induced in male mice following 8 weeks of subchronic exposure to di-n-butyl phthalate (DBP) during their puberty and to investigate the possibility of transmission of mutations to subsequent generations via the sperm. Pzh:Sfis outbred male mice aged 4.5 weeks were exposed to DBP by gavage for 8 weeks, 3 days per week to doses of 1/16 LD50 or 1/4 LD50 each time. Six to seven males from each dosage group were sacrificed at 4, 8 and 12 weeks after the start of exposure for examination of sperm count and quality. Immediately after the end of exposure, the remaining males were caged for 1 week with two unexposed females each. Group of females were sacrificed 1 day before expected parturition, whilst other females were allowed to deliver and rear litters. F1 generation males at 8-9 weeks of age were caged with females from the same group, but from a different litter, for examination of prenatal development of the F2 generation. The remaining F1 generation males were sacrificed at the same age to check the sperm count and quality. Our results confirmed the toxic effects of DBP on the reproductive organs and germ cells of pubertally exposed males. The changes induced in male gametes might be transmitted to the next generation via the sperm. The most important effects were induced in the F1 generation. Exposure of F0 males to DBP induced skeletal malformations in surviving foetuses, caused significant mortality in postnatal life and a disturbance in the sex ratio (superior survival of females in F1), as well as increased frequency of DNA damage in the germ cells of F1 males. The present study did not confirm higher sensitivity to DBP of pubescent males compared to adult males, but the effects induced in the F1 generation differed from that after exposure of adult F0 males.
Keywords
Animals; Animals, Outbred Strains; Dibutyl Phthalate/toxicity; Infertility, Male/chemically induced; Mutagens/toxicity; Sexual Maturation/drug effects; Sperm Count; Sperm Motility; Testis/drug effects/growth & development/pathology; 2286E5R2KE
Tags
IRIS
•
Dibutyl Phthalate (DBP)
Database Searches
LitSearch Jan 2017 - July 2017
Pubmed
WOS
LitSearch July 2017 - Sept 2018
Prior Search Overlap
WOS
Studies with Supporting Data
Mechanistic and genotoxicity studies
Studies with Health Effects Data
Animal toxicology studies
•
Phthalates – Targeted Search for Epidemiological Studies
Source – all searches
Pubmed
WOS
Excluded
Source - August 2017 Update (Private)
Pubmed
WOS
Source - August 2018 Update
WOS
Level 1 Screen - Title & Abstract
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