Separation of underivatized small peptides on a porous graphitic carbon column by ion-pair chromatography and evaporative light scattering detection | Health & Environmental Research Online (HERO)

Health & Environmental Research Online (HERO)

Print Feedback Export to File

This record has one attached file:

Citation
Tags

HERO ID

3859447

Reference Type

Journal Article

Title

Separation of underivatized small peptides on a porous graphitic carbon column by ion-pair chromatography and evaporative light scattering detection

Author(s)

Adoubel, AA; Guenu, S; Elfakir, C; Dreux, M

Year

2000

Is Peer Reviewed?

Yes

Journal

Journal of Liquid Chromatography and Related Technologies
ISSN: 1082-6076
EISSN: 1520-572X

Volume

Issue

Page Numbers

2433-2446

Language

English

DOI

10.1081/JLC-100100499

Web of Science Id

WOS:000089755000001

Abstract

Separations of small peptides (di-, tri- and tetrapeptides) using ion-pair chromatography and evaporative light scattering detection have been investigated with a porous graphitic carbon stationary phase, Hypercarb(R). Five perfluorinated carboxylic acids (trifluoroacetic (TFA), pentafluoropropanoic (PFPA), heptafluorobutanoic (HFBA), nonafluoropentanoic (NFPA) and tridecafluoroheptanoic (TDFHA) acids) have been evaluated as ion-pairing agents. Influence of the percentage of organic modifier, concentration, and chain length of the ion-pairing agent on the retention factors, k, has been examined. Equilibration times were also studied. Separations of dipeptides or tri- and tetrapeptides are presented using HFBA and NFPA at 20 mM and TDFHA at 2.5 mM.

Tags