US EPA

3860238 

Journal Article 

Production of Antimicrobial Metabolites by Streptomyces lavendulocolor VHB-9 Isolated from Granite Mines 

Bindu, HBSSN; Muvva, V; Munaganti, RK; Naragani, K; Konda, S; Dorigondla, KR 

2017 

Yes 

Brazilian Archives of Biology and Technology
ISSN: 1516-8913
EISSN: 1678-4324 

INST TECNOLOGIA PARANA 

CURITIBA-PARANA 

60 

60385-60385 

English 

10.1590/1678-4324-2017160385 

WOS:000404477500001 

http://://CCC:000404477500001
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The actinobacterial strain Streptomyces lavendulocolor VHB-9 was isolated from granite mine soil samples of Khammam district, Telangana state, India. The strain was identified based on detailed microorphological, cultural and phylogenetic analysis. Bioactive guided isolation of the secondary metabolites of the strain was carried out by growing the strain in optimized medium (0.5% lactose, 0.5% peptone, 0.05% K2HPO4, 0.2% CaCO3 with pH adjusted to 7.0). Separation and purification of the active fractions from the crude ethyl acetate extract was carried out by silica gel column chromatography and resulted in the isolation of two active fractions. Structural elucidation of the two (B2 and B3) active compounds was carried out by FT-IR, Mass and NMR spectroscopy and were identified as Bis (7-methyloctyl) phthalate and (Z)-3-aminoacrylic acid. The antimicrobial activity of the bioactive compounds produced by S. lavendulocolor VHB-9 was expressed in terms of minimum inhibitory concentration against opportunistic pathogenic bacteria and fungi. Both fractions exhibited good antimicrobial potential against the bacteria and fungi tested. 

Granite; Streptomyces lavendulocolor; spectroscopy; Bis (7-methyloctyl) phthalate; (Z)-3-aminoacrylic acid