US EPA

3860581 

Journal Article 

Determination of cocaine and benzoylecgonine by derivatization with iodomethane-D3 or PFPA/HFIP in human blood and urine using GC/MS (EI or PCI mode) 

Aderjan, RE; Schmitt, G; Wu, M; Meyer, C 

1993 

Yes 

Journal of Analytical Toxicology
ISSN: 0146-4760
EISSN: 1945-2403 

17 

1 

51-55 

English 

8429629 

10.1093/jat/17.1.51 

WOS:A1993KH43400013 

A method for the simultaneous detection and quantification of cocaine and benzoylecgonine from human blood and urine has been developed. Cocaine and benzoylecgonine were extracted from blood and urine with solid-phase columns containing C18 packing material. Clean, emulsion-free extracts were obtained. The analytes were derivatized with PFPA/HFIP or iodomethane-D3. The underivatized cocaine and derivatized benzoylecgonine were determined by GC/MS with positive chemical ionization (PCI) and the selected ion monitoring mode. Benzoylecgonine was derivatized with iodomethane-D3 to cocaine-D3. Both derivatives were chromatographed on a CP-SIL 5 capillary column. The recovery of extraction was greater than 70%. The detection limit for the PFPA/HFIP derivatives (20 ng/mL) was better than that for iodomethane-D3 derivatives (40 ng/mL). The procedure is simple, rapid, and reproducible. Standard curves were linear and the procedure was suitable for routine analysis. Using fluorescence polarization immunoassay (TDx, Abbott) (cutoff 100 ng/mL), along with one of the GC/MS methods described, we found no false positive or false negative results. 

1-Propanol/chemistry; Cocaine/analogs & derivatives/blood/urine; Fluorescence Polarization Immunoassay; Fluorocarbons/chemistry; Gas Chromatography-Mass Spectrometry; Hydrocarbons, Iodinated/chemistry; Propanols; Substance Abuse Detection/methods