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HERO ID
3873282
Reference Type
Journal Article
Title
Coal combustion related fine particulate matter (PM2.5) induces toxicity in Caenorhabditis elegans by dysregulating microRNA expression
Author(s)
Wu, Q; Han, X; Wang, Di; Zhao, F; Wang, D
Year
2017
Is Peer Reviewed?
1
Journal
Toxicology Research
ISSN:
2045-452X
EISSN:
2045-4538
Volume
6
Issue
4
Page Numbers
432-441
Language
English
PMID
30090511
DOI
10.1039/c7tx00107j
Web of Science Id
WOS:000404873500005
Abstract
We employed an in vivo assay system of Caenorhabditis elegans to determine if and which microRNAs (miRNAs) were dysregulated upon exposure to coal combustion related fine particulate matter (PM2.5) by profiling the miRNAs using SOLiD sequencing. From this, expression of 25 miRNAs was discovered to become dysregulated by exposure to PM2.5. Using the corresponding C. elegans deletion mutants, 5 miRNAs (mir-231, mir-232, mir-230, mir-251 and mir-35) were found to be involved in the control of PM2.5 toxicity. Furthermore, mutation of mir-231 or mir-232 induced a resistance to PM2.5 toxicity, whereas mutation of mir-230, mir-251, or mir-35 induced a susceptibility to PM2.5 toxicity. SMK-1, an ortholog of the mammalian SMEK protein, was identified as a molecular target for mir-231 in the regulation of PM2.5 toxicity. In addition, the genes of sod-3, sod-4 and ctl-3, which are necessary for protection against oxidative stress, were determined to be important downstream targets of smk-1 in the regulation of PM2.5 toxicity. The triggering of this mir-231-SMK-1-SOD-3/SOD-4/CTL-3 signaling pathway may be a critical molecular basis for the role of oxidative stress in the induction of coal combustion related PM2.5 toxicity.
Tags
NAAQS
•
ISA NOxSOxPM Ecology (2018)
Cited in the Second Draft
Appendix 15
•
ISA NOxSOxPM Ecology (2020- Final Project Page)
Cited
Appendix 15
•
Litsearch – PM ISA Supplement 2021
Pubmed iCite citation search (April 2021 BR)
PM2.5 Cardiovascular and Mortality Epi Search
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