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Citation
Tags
HERO ID
3982675
Reference Type
Journal Article
Title
Lactational exposure effect of polychlorinated biphenyl on rat sertoli cell markers and functional regulators in prepuberal and puberal F1 offspring
Author(s)
Priya, ES; Kumar, TS; Balaji, S; Bavithra, S; Singh, PR; Sakthivel, D; Sankar, BR; Arunakaran, J
Year
2017
Is Peer Reviewed?
Yes
Journal
Journal of Endocrinological Investigation
ISSN:
0391-4097
EISSN:
1720-8386
Volume
40
Issue
1
Page Numbers
91-100
Language
English
PMID
27614457
DOI
10.1007/s40618-016-0539-0
Web of Science Id
WOS:000391430700013
Relationship(s)
is supplemented by
5020841
Supplemental materials:
Abstract
PURPOSE:
Polychlorinated biphenyls (PCBs) are persistent and bioaccumulative environmental toxicants acting as endocrine disruptors. Many researches evidenced that PCBs affect the male reproductive system in adult rats and it can transfer from mother to offspring through milk. We investigated whether the lactational exposure to PCBs affects the Sertoli cell function in F1 offspring.
METHODS:
Dams were orally treated with different doses of PCB-Aroclor 1254 (1, 2 and 5 mg/kg bw/day, respectively) from postpartum day 1-20. Male offspring rats were killed on PND 21 and PND 60. Testes were used both for histological study and to isolate Sertoli cell. Serum and testicular interstitial fluid (TIF) levels of testosterone, ABP and estradiol were analyzed by ELISA method. The mRNA and protein expressions of follicle-stimulating hormone (FSHR), androgen-binding protein (ABP), Inhibinβ, androgen receptor (AR) and estrogen receptor (ERβ) were studied using real-time PCR and immunoblotting, respectively.
RESULTS:
The testicular architecture was altered in PCB-treated groups of both prepuberal and puberal rats. Testosterone, estradiol and androgen-binding protein levels were altered in both serum and TIF in PCB treated groups. The gene expression level of FSHR, ABP, ERβ and AR was decreased in a dose-dependent manner, whereas Inhibinβ gene expression level was increased in PCB-treated groups.
CONCLUSION:
Lactational exposure to PCB affects both the histoarchitecture of testis, Sertoli cell maker and functional regulators in both prepuberal and puberal F1 male progeny.
Tags
IRIS
•
PCBs
Mechanistic
Supplemental
Litsearches
LitSearch: August 2016-August 2017
PubMed
Toxline
WoS
•
PCBs Tox Hazard ID
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