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Citation
Tags
HERO ID
3982697
Reference Type
Journal Article
Title
Congener-Specific Disposition of Chiral Polychlorinated Biphenyls in Lactating Mice and Their Offspring: Implications for PCB Developmental Neurotoxicity
Author(s)
Kania-Korwel, I; Lukasiewicz, T; Barnhart, CD; Stamou, M; Chung, H; Kelly, KM; Bandiera, S; Lein, PJ; Lehmler, HJ
Year
2017
Is Peer Reviewed?
1
Journal
Toxicological Sciences
ISSN:
1096-6080
EISSN:
1096-0929
Volume
158
Issue
1
Page Numbers
101-115
Language
English
PMID
28431184
DOI
10.1093/toxsci/kfx071
Web of Science Id
WOS:000405698800009
Abstract
Chiral polychlorinated biphenyl (PCB) congeners have been implicated by laboratory and epidemiological studies in PCB developmental neurotoxicity. These congeners are metabolized by cytochrome P450 (P450) enzymes to potentially neurotoxic hydroxylated metabolites (OH-PCBs). The present study explores the enantioselective disposition and toxicity of two environmentally relevant, neurotoxic PCB congeners and their OH-PCB metabolites in lactating mice and their offspring following dietary exposure of the dam. Female C57BL/6N mice (8 weeks old) were fed daily, beginning two weeks prior to conception and continuing throughout gestation and lactation, with 3.1 µmol/kg bw/d of racemic 2,2',3,5',6-pentachlorobiphenyl (PCB 95) or 2,2',3,3',6,6'-hexachlorobiphenyl (PCB 136) in peanut butter; controls received vehicle (peanut oil) in peanut butter. PCB 95 levels were higher than PCB 136 levels in both dams and pups, consistent with the more rapid metabolism of PCB 136 compared to PCB 95. In pups and dams, both congeners were enriched for the enantiomer eluting second on enantioselective gas chromatography columns. OH-PCB profiles in lactating mice and their offspring were complex and varied according to congener, tissue and age. Developmental exposure to PCB 95 vs. PCB 136 differentially affected the expression of P450 enzymes as well as neural plasticity (arc and ppp1r9b) and thyroid hormone-responsive genes (nrgn and mbp). The results suggest that the enantioselective metabolism of PCBs to OH-PCBs may influence neurotoxic outcomes following developmental exposures, a hypothesis that warrants further investigation.
Tags
IRIS
•
PCBs
Mechanistic
ADME and pharmacokinetic modeling
Supplemental
Litsearches
LitSearch: August 2016-August 2017
PubMed
Toxline
WoS
•
PCBs Tox Hazard ID
Administered mixture of < 4 individual PCB congeners
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