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HERO ID
3985579
Reference Type
Journal Article
Title
Effects of Aroclor 1254 on the DNA methylation of imprinted genes in the adult mouse sperm
Author(s)
Wang, CY; Cui, JT; Lu, ZL; Ma, XL; Wang, JH; Sun, YC
Year
2017
Is Peer Reviewed?
Yes
Journal
Applied Ecology and Environmental Research
ISSN:
1589-1623
EISSN:
1785-0037
Volume
15
Issue
3
Page Numbers
999-1012
Language
English
DOI
10.15666/aeer/1503_9991012
Web of Science Id
WOS:000403811200073
Abstract
As estrogen-like and anti- androgenic endocrine-disrupting chemicals, polychlorinated biphenyls have been reported to affect the male reproductive axis resulting in infertility. In the present study, Aroclor 1254 was administered to adult male mice, and its possible effects on the differentially methylated regions of two paternally (H19 and Gtl2) and three maternally (Mest, Snrpn and Igf2r) imprinted genes were tested in the sperm DNA. In the control, the percentages of methylated CpGs were close to 100% and 0% in paternally or maternally imprinted genes respectively. Aroclor 1254 significantly decreased the percentages of methylated CpGs of H19 and Gtl2 and markedly increased those of Mest, Snrpn, and Igf2r. In addition, dramatically increased mRNA expression of estrogen receptor alpha and beta were obtained in the treatment. We postulate that the effects of PCBs on male spermatogenesis and fertility rates might involve imprinting alterations in the sperm.
Keywords
polychlorinated biphenyls; endocrine disrupting chemicals; epigenetics; CpG island; sperm cell
Tags
IRIS
•
PCBs
Mechanistic
ADME and pharmacokinetic modeling
Supplemental
Litsearches
LitSearch: August 2016-August 2017
WoS
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