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HERO ID
4238289
Reference Type
Journal Article
Title
Single-Droplet Multiplex Bioassay on a Robust and Stretchable Extreme Wetting Substrate through Vacuum-Based Droplet Manipulation
Author(s)
Han, H; Lee, JS; Kim, H; Shin, S; Lee, J; Kim, J; Hou, X; Cho, SW; Seo, J; Lee, T
Year
2018
Is Peer Reviewed?
1
Journal
ACS Nano
ISSN:
1936-0851
EISSN:
1936-086X
Volume
12
Issue
2
Page Numbers
932-941
Language
English
PMID
29262253
DOI
10.1021/acsnano.7b05826
Abstract
Herein, a droplet manipulation system with a superamphiphobic (SPO)-superamphiphilic (SPI) patterned polydimethylsiloxane (PDMS) substrate is developed for a multiplex bioassay from single-droplet samples. The SPO substrate is fabricated by sequential spraying of adhesive and fluorinated silica nanoparticles onto a PDMS substrate. It is subsequently subjected to oxygen plasma with a patterned mask to form SPI patterns. The SPO layer exhibits extreme liquid repellency with a high contact angle (>150°) toward low surface tension and viscous biofluidic droplets (e.g., ethylene glycol, blood, dimethyl sulfoxide, and alginate hydrogel). In contrast, the SPI exhibits liquid adhesion with a near zero contact angle. Using the droplet manipulation system, various liquid droplets can be precisely manipulated and dispensed onto the predefined SPI patterns on the SPO PDMS substrate. This system enables a multiplex colorimetric bioassay, capable of detecting multiple analytes, including glucose, uric acid, and lactate, from a single sample droplet. In addition, the detection of glucose concentrations in a plasma droplet of diabetic and healthy mice are performed to demonstrate the feasibility of the proposed system for efficient clinical diagnostic applications.
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