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HERO ID
4631388
Reference Type
Journal Article
Title
Studies on the susceptibility of various organs of zebrafish (Brachydanio rerio) to benzo(a)pyrene-induced DNA adduct formation
Author(s)
Hsu, T; Deng, FY
Year
1996
Is Peer Reviewed?
Yes
Journal
Chemosphere
ISSN:
0045-6535
EISSN:
1879-1298
Publisher
Elsevier Ltd
Volume
33
Issue
10 (1996)
Page Numbers
1975-1980
Language
English
DOI
10.1016/0045-6535(96)00299-8
Web of Science Id
WOS:A1996VT31400009
Abstract
The relative susceptibility of zebrafish (Brachydanio rerio) organs (liver, intestine, brain, testis) to DNA adduct formation induced by exposure to waterborne benzo(a)pyrene (BaP) at a concentration of 0.2 mg per litre for 3 d and a concentration of 1 mg per litre for 4 d was investigated using phosphorus-32 post-labelling analysis. Autoradiography of TLC chromatograms was used to identify the extent of DNA adduct formation. The number of BaP adducts in the intestine was 3 times that in the liver and the average adduct level in brain DNA was only slightly lower than liver DNA. No adduct spots were detected in DNA isolated from control fish organs. The intestine of zebrafish was more sensitive to the presence of toxic BaP metabolites than the liver, especially when PAH were present at low levels.
Keywords
benzo(a)pyrene; fish; DNA adduct; P-32-postlabeling
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