Studies on the susceptibility of various organs of zebrafish (Brachydanio rerio) to benzo(a)pyrene-induced DNA adduct formation
Hsu, T; Deng, FY
| HERO ID | 4631388 |
|---|---|
| In Press | No |
| Year | 1996 |
| Title | Studies on the susceptibility of various organs of zebrafish (Brachydanio rerio) to benzo(a)pyrene-induced DNA adduct formation |
| Authors | Hsu, T; Deng, FY |
| Journal | Chemosphere |
| Volume | 33 |
| Issue | 10 (1996) |
| Page Numbers | 1975-1980 |
| Abstract | The relative susceptibility of zebrafish (Brachydanio rerio) organs (liver, intestine, brain, testis) to DNA adduct formation induced by exposure to waterborne benzo(a)pyrene (BaP) at a concentration of 0.2 mg per litre for 3 d and a concentration of 1 mg per litre for 4 d was investigated using phosphorus-32 post-labelling analysis. Autoradiography of TLC chromatograms was used to identify the extent of DNA adduct formation. The number of BaP adducts in the intestine was 3 times that in the liver and the average adduct level in brain DNA was only slightly lower than liver DNA. No adduct spots were detected in DNA isolated from control fish organs. The intestine of zebrafish was more sensitive to the presence of toxic BaP metabolites than the liver, especially when PAH were present at low levels. |
| Doi | 10.1016/0045-6535(96)00299-8 |
| Wosid | WOS:A1996VT31400009 |
| Is Certified Translation | No |
| Dupe Override | 4631388 |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030297665&doi=10.1016%2f0045-6535%2896%2900299-8&partnerID=40&md5=e3d8af789244aea0fcad521a73ce2aac |
| Is Public | Yes |
| Language Text | English |
| Keyword | benzo(a)pyrene; fish; DNA adduct; P-32-postlabeling |
| Relationship(s) |
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