Studies on the susceptibility of various organs of zebrafish (Brachydanio rerio) to benzo(a)pyrene-induced DNA adduct formation | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

4631388

Reference Type

Journal Article

Title

Studies on the susceptibility of various organs of zebrafish (Brachydanio rerio) to benzo(a)pyrene-induced DNA adduct formation

Author(s)

Hsu, T; Deng, FY

Year

1996

Is Peer Reviewed?

Yes

Journal

Chemosphere
ISSN: 0045-6535
EISSN: 1879-1298

Publisher

Elsevier Ltd

Volume

Issue

10 (1996)

Page Numbers

1975-1980

Language

English

DOI

10.1016/0045-6535(96)00299-8

Web of Science Id

WOS:A1996VT31400009

Abstract

The relative susceptibility of zebrafish (Brachydanio rerio) organs (liver, intestine, brain, testis) to DNA adduct formation induced by exposure to waterborne benzo(a)pyrene (BaP) at a concentration of 0.2 mg per litre for 3 d and a concentration of 1 mg per litre for 4 d was investigated using phosphorus-32 post-labelling analysis. Autoradiography of TLC chromatograms was used to identify the extent of DNA adduct formation. The number of BaP adducts in the intestine was 3 times that in the liver and the average adduct level in brain DNA was only slightly lower than liver DNA. No adduct spots were detected in DNA isolated from control fish organs. The intestine of zebrafish was more sensitive to the presence of toxic BaP metabolites than the liver, especially when PAH were present at low levels.

Keywords

benzo(a)pyrene; fish; DNA adduct; P-32-postlabeling