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HERO ID
4829302
Reference Type
Journal Article
Title
Nontargeted metabolomic analysis to unravel the impact of di (2-ethylhexyl) phthalate stress on root exudates of alfalfa (Medicago sativa)
Author(s)
Wang, Y; Ren, W; Li, Y; Xu, Y; Teng, Y; Christie, P; Luo, Y
Year
2019
Is Peer Reviewed?
1
Journal
Science of the Total Environment
ISSN:
0048-9697
EISSN:
1879-1026
Publisher
Elsevier B.V.
Location
AMSTERDAM
Volume
646
Page Numbers
212-219
Language
English
PMID
30053665
DOI
10.1016/j.scitotenv.2018.07.247
Web of Science Id
WOS:000445164800023
URL
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050284381&doi=10.1016%2fj.scitotenv.2018.07.247&partnerID=40&md5=8760c0ae8f1d7247d9c04ea089c8dff9
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Abstract
Root exudates are the main media of information communication and energy transfer between plant roots and the soil. Understanding the response of root exudates to contamination stress is crucial in revealing the rhizoremediation mechanisms. Here, we investigate the response of alfalfa root exudates to bis(2-ethylhexyl) phthalate (DEHP) stress based on nontargeted metabolomic analysis. Alfalfa root exudates were collected using greenhouse hydroponic culture and analysed by gas chromatography-time of flight mass spectrometry (GC-TOFMS). A total of 314 compounds were identified in alfalfa root exudates of which carbohydrates, acids and lipids accounted for 28.6, 15.58 and 13.87%, respectively. Orthogonal partial least squares discriminant analysis (OPLS-DA) shows that DEHP exerted an important influence on the composition and quantity of root exudates. Fifty metabolites were clearly changed even at lower concentrations of DEHP, including common carbohydrates, fatty acids and some special rhizosphere signal materials, such as 4',5-dihyrroxy-7-methoxyisoflavone. DEHP stress significantly suppressed carbohydrate metabolism but promoted fatty acid metabolism. However, amino acid metabolism, lipid metabolism and the tricarboxylic acid (TCA) cycle showed little change in response to DEHP stress.
Keywords
Diethylhexyl Phthalate/toxicity; Medicago sativa/chemistry/drug effects; Phthalic Acids; Plant Exudates/metabolism; Plant Roots; Rhizosphere; Soil Pollutants/toxicity; Alfalfa; DEHP stress; Metabolomics; Rhizoremediation; Root exudates; 6O7F7IX66E; C42K0PH13C
Tags
IRIS
•
Dibutyl Phthalate (DBP)
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