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HERO ID
4850289
Reference Type
Journal Article
Title
Nano-sized selenium attenuates the developmental testicular toxicity induced by di-n-butyl phthalate in pre-pubertal male rats
Author(s)
Rashad, MM; Galal, MK; Abou-El-Sherbini, KS; El-Behairy, AM; Gouda, EM; Moussa, SZ
Year
2018
Is Peer Reviewed?
Yes
Journal
Biomedicine & Pharmacotherapy
ISSN:
0753-3322
EISSN:
1950-6007
Volume
107
Page Numbers
1754-1762
Language
English
PMID
30257394
DOI
10.1016/j.biopha.2018.09.006
Web of Science Id
WOS:000445036200192
URL
https://search.proquest.com/docview/2113281556?accountid=171501
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Abstract
The current study was conducted to test the possible ameliorative role of selenium nanoparticles (Se-NPs) against oxidative damage of Leyding cells induced by di-n-butyl phthalate (DBP) in pre-pubertal male rat offspring. Forty-two pregnant female rats treated from gestation day (GD) 12 to postnatal day (PND) 14 day with two doses of Se-NPs (0.2 and 0.5 mg/kg/d) against developmental testicular toxicity induced by DBP (500 mg/kg/d). At PND 25 serum and testes of offspring were collected. Serum LH, the Leydig cells performance [total serum testosterone, LH and testosterone (LH/T) ratio, relative gene expression of insulin-like growth factor-3 (INSL3) and mineralocorticoid receptor (MR)], oxidative stress biomarker malondialdehyde (MDA) and antioxidant machinery [reduced glutathione (GSH), and the relative gene expression of antioxidant enzymes: superoxide dismutase (SOD), glutathione peroxidase (GPx)] were estimated in all groups. The obtained results revealed that maternal exposure to DBP significantly reduced total serum testosterone level, relative mRNA expression of INSL3 and MR genes with observed testicular damage revealed by increasing MDA and depressed levels of GSH and antioxidant enzymes. The histopathological changes include necrosis and desquamation of spermatogoneal cells. Co-administration of Se-NPs high dose along with DBP significantly increased serum testosterone, improved LH/T ratio and the relative mRNA expression of INSL3 and MR genes, decreased the level of MDA, and also improved all the antioxidant enzymes expression levels. In conclusion, Se-NPs could be a potent maternal prophylactic agent against the reduced total serum testosterone level and oxidative damage of Leydig cells induced by DBP via reducing the lipid peroxidation (LPO) and enhancing the antioxidant state in pre-pubertal male rat offspring.
Keywords
Antioxidants; Insulin; Leydig insulin-like protein; Proteins; RNA, Messenger; Dibutyl Phthalate; 2286E5R2KE; Testosterone; 3XMK78S47O; Malondialdehyde; 4Y8F71G49Q; Luteinizing Hormone; 9002-67-9; Glutathione Peroxidase
Tags
IRIS
•
Dibutyl Phthalate (DBP)
Additional Strategies
Studies with Supporting Data
Mechanistic and genotoxicity studies
Studies with Health Effects Data
Animal toxicology studies
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