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5080569 
Journal Article 
Effect of perfluorodecanoic acid on pig oocyte viability, intracellular calcium levels and gap junction intercellular communication during oocyte maturation in vitro 
Domínguez, A; Salazar, Z; Betancourt, M; Ducolomb, Y; Casas, E; Fernández, F; Bahena, I; Salomón, A; Teteltitla, M; Martínez, R; Chaparro, A; Cuapio, P; Salazar-López, C; Bonilla, E 
2019 
Yes 
Toxicology In Vitro
ISSN: 0887-2333
EISSN: 1879-3177 
58 
224-229 
English 
Perfluorodecanoic acid (PFDA) is a synthetic perfluorinated compound, which has been reported to exert adverse effects on somatic cells. However, its effects on germ cells have not been studied to date. The aim of the present study was to analyze the effects of PFDA on the viability, intracellular calcium levels and gap junction intercellular communication (GJIC) during porcine oocyte maturation in vitro. PFDA negatively impacted oocyte viability (medium lethal concentration, LC50 = 7.8 μM) and maturation (medium inhibition of maturation, IM50 = 3.8 μM). Oocytes exposed to 3.8 μM PFDA showed higher levels of intracellular calcium relative to control oocytes. In addition, GJIC among the cumulus cells and the oocyte was disrupted. The effects of PFDA on oocyte calcium homeostasis and intercellular communication seem to be responsible for the inhibition of oocyte maturation and oocyte death. In addition, since the deleterious effects of PFDA on oocyte viability, maturation and GJIC are significantly stronger than the previously reported effects of another widely used perfluorinated compound (Perfluorooctane sulfonate) in the same model, the use of PFDA in consumer products is questioned. 
PFDA; Oocyte maturation; Intracellular calcium; GJIC; Pig 
• PFDA
     Litsearch Update 5/2019
          PubMed
• PFOA (335-67-1) and PFOS (1763-23-1)
     Literature Search Update (2013-2019)
          PubMed