US EPA

5083623 

Journal Article 

Perfluorononanoic acid (PFNA) alters lipid accumulation in bovine blastocysts after oocyte exposure during in vitro maturation 

Hallberg, I; Kjellgren, J; Persson, S; Örn, S; Sjunnesson, Y 

2019 

1 

Reproductive Toxicology
ISSN: 0890-6238
EISSN: 1873-1708 

84 

1-8 

English 

30502403 

10.1016/j.reprotox.2018.11.005 

WOS:000462417700001 

https://search.proquest.com/docview/2149016535?accountid=171501
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Perfluorononanoic acid (PFNA) is one of the perfluoroalkyl acids present in human tissues. In this study, effects on early embryo development after PFNA exposure were investigated using the bovine in vitro production system. Oocytes were exposed to PFNA during maturation in vitro (10 μg mL-1 and 0.1 μg mL-1), and then fertilized and cultured in parallel with control groups. Developmental parameters (cleavage, blastocyst formation) were followed and embryo quality evaluated (stage, grade). Embryos developed after exposure to 0.1 μg mL-1 were stained to distinguish nuclei, active mitochondria and neutral lipids. 10 μg mL-1 of PFNA had a severe negative effect on blastocyst formation (OR: 0.27 p < 0.05), an effect not observed at 0.1 μg mL-1. However, lipid droplet distribution was significantly altered in embryos exposed to 0.1 μg mL-1, suggesting a disturbance of lipid metabolism after exposure to sublethal levels of PFNA during oocyte maturation in vitro. 

Fluorocarbons; perfluorononanoic acid; 5830Z6S63M; Index Medicus; Image analysis; Developmental toxicology; Lipid droplet; Endocrine disruption; Animals; Fertilization in Vitro; Embryonic Development -- drug effects; Oocytes -- drug effects; Oocytes -- physiology; In Vitro Oocyte Maturation Techniques; Embryo, Mammalian -- physiology; Blastocyst -- physiology; Embryo, Mammalian -- drug effects; Fluorocarbons -- toxicity; Blastocyst -- drug effects; Lipid Metabolism -- drug effects