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Citation
Tags
HERO ID
5099481
Reference Type
Journal Article
Title
The role of fluoroalcohols as counter anions for ion-pairing reversed-phase liquid chromatography/high-resolution electrospray ionization mass spectrometry analysis of oligonucleotides
Author(s)
Liu, R; Ruan, Y; Liu, Z; Gong, L
Year
2019
Is Peer Reviewed?
1
Journal
Rapid Communications in Mass Spectrometry
ISSN:
0951-4198
EISSN:
1097-0231
Volume
33
Issue
7
Page Numbers
697-709
Language
English
PMID
30668892
DOI
10.1002/rcm.8386
Web of Science Id
WOS:000462668100009
Abstract
RATIONALE:
Hexafluoroisopropanol (HFIP) has been widely used as a counter anion in the mobile phase for ion-pairing reversed-phase liquid chromatography/mass spectrometry (IP-RP-LC/MS) analysis of oligonucleotides. However, researchers are still searching for improvements to counter anions for LC/MS analysis of oligonucleotides. This study aimed to find alternatives to HFIP for analyzing oligonucleotides.
METHODS:
The study was performed using an Agilent 1290 ultra-high-performance liquid chromatography (UHPLC) system coupled to an Agilent 6540 mass spectrometer by using an oligonucleotide BEH C18 column (100 × 2.1 mm, 1.7 μm). Buffer systems containing ion-pairing reagents (triethylamine, tripropylamine, hexylamine, dimethylbutylamine, diisopropylethylamine, N,N-dimethylcyclohexylamine, and octylamine) and fluoroalcohols (HFIP and hexafluoro-2-methyl-2-propanol (HFTP)) were compared chromatographically and mass spectrometrically.
RESULTS:
Results showed that HFTP has better desalting ability than HFIP, but both HFIP and HFTP have comparable effects on the separation of oligonucleotides sized from 10mer to 40mer for most of ion-pairing reagents, with the exception of triethylamine and N,N-dimethylcyclohexylamine, where HFIP performed better than HFTP.
CONCLUSIONS:
The choice of fluoroalcohols in IP-RP-LC/MS analysis of oligonucleotides depends on the type of ion-pairing reagents used in the mobile phase. As a guideline, we would recommend to use either HA-HFIP or HA-HFTP for small oligonucleotides, but TPA-HFTP for large oligonucleotides for IP-RP-LC/MS analysis of synthetic oligonucleotides.
Tags
IRIS
•
tert-Butanol
Excluded/ Not on Topic
Chemical Analysis/Fuel Chemistry
LitSearch: Jan 2017 - July 2019
WoS
PubMed
PFAS
•
Expanded PFAS SEM (formerly PFAS 430)
Litsearch: September 2019
PubMed
Web of Science
Not prioritized for screening
2,3-Dichlorooctafluorobutane
2H-Perfluoro-2-propanol
Hexafluoro-2-methyl-2-propanol
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