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HERO ID
5680627
Reference Type
Journal Article
Title
In utero exposure of high-dose di-n-butyl phthalate resulted in opposite effects on testicular cell apoptosis in late embryonic and pubertal male rat offspring
Author(s)
Shen, H; Liao, K; Wu, HF; Lu, HC; Li, Y; Li, Z; Zhang, W
Year
2017
Is Peer Reviewed?
Yes
Journal
Human & Experimental Toxicology
ISSN:
0960-3271
EISSN:
1477-0903
Volume
36
Issue
12
Page Numbers
1236-1247
Language
English
PMID
28067064
DOI
10.1177/0960327116685886
Web of Science Id
WOS:000414993600002
URL
https://search.proquest.com/docview/1967325729?accountid=171501
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Abstract
Objective: To investigate the effects of in utero exposure to high-dose di-n -butyl phthalate (DBP) on testicular cell apoptosis in late embryonic and pubertal male rat offspring. Methods: Twenty pregnant Sprague-Dawley (SD) rats were divided into two groups. During gestation day (GD) 12 to GD 19, control group was given 1 ml day-1 of olive oil and experimental group was given DBP 500 mg kg-1 day-1 by gavage. On GD 19.5 and postnatal day (PND) 45, the testes were removed. Morphological analysis of the testes was observed by transmission electron microscopy and hematoxylin and eosin (H&E) staining. Testicular cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). The expression of Bcl-2, Bax, and p53 was presented by immunohistochemistry (IHC) and western blot. Data of the two groups was compared using independent samples t -test and Mann-Whitney test by SPSS 20.0. Results: H&E staining showed that spermatogenetic cells were significantly decreased in DBP exposed pubertal rat testis. The apoptosis index of testes in DBP-treated group was significantly lower on GD 19.5 but higher on PND 45 than that of the controls (p < 0.01). IHC and western blot revealed significantly increased expression of Bcl-2 in GD 19.5 rat testis and Bax and p53 in PND 45 rat testis after DBP exposure, compared with the control (p < 0.05). Conclusion: In utero exposure of high-dose DBP resulted in opposite effects on testicular cell apoptosis in late embryonic and pubertal rat offspring. The overexpression of Bcl-2, Bax, and p53 might be related to the occurrence of abnormal apoptosis and finally produce male infertility.
Keywords
Pharmacy And Pharmacology; Testicular dysgenesis; apoptosis; di-n-butyl phthalate; Intrauterine exposure; p53 Protein; Pregnancy; n-Butyl phthalate; Bax protein; Bcl-2 protein; Fats and oils; Exposure; Rodents; Offspring; DNA nucleotidylexotransferase; Butyl phthalate; Embryos; Electron microscopy; Gestation; Infertility; Staining; Immunohistochemistry; Transmission electron microscopy; Olive oil; Progeny
Tags
IRIS
•
Dibutyl Phthalate (DBP)
Database Searches
LitSearch Jul 2016 - Jan 2017
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PubMed
LitSearch July 2017 - Sept 2018
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WOS
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Phthalates – Targeted Search for Epidemiological Studies
Source - August 2018 Update
Pubmed
Toxline
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