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Citation
Tags
HERO ID
6324256
Reference Type
Journal Article
Title
Investigation of the Interaction Mechanism of Perfluoroalkyl Carboxylic Acids with Human Serum Albumin by Spectroscopic Methods
Author(s)
Chen, H; Wang, Q; Cai, Y; Yuan, R; Wang, F; Zhou, B
Year
2020
Is Peer Reviewed?
Yes
Journal
International Journal of Environmental Research and Public Health
ISSN:
1661-7827
EISSN:
1660-4601
Publisher
MDPI AG
Volume
17
Issue
4
Language
English
PMID
32085632
DOI
10.3390/ijerph17041319
Web of Science Id
WOS:000522388500195
Abstract
Perfluoroalkyl carboxylic acids (PFCAs) are some of the most significant pollutants in human serum, and are reported to be potentially toxic to humans. In this study, the binding mechanism of PFCAs with different carbon lengths to human serum albumin (HSA) was studied at the molecular level by means of fluorescence spectroscopy under simulated physiological conditions and molecular modeling. Fluorescence data indicate that PFCAs with a longer carbon chain have a stronger fluorescence quenching ability. Perfluorobutanoic acid (PFBA) and perfluorohexanoic acid (PFHxA) had little effect on HSA. Fluorescence quenching of HSA by perfluorooctanoic acid (PFOA) and perfluorodecanoic acid (PFDA) was a static process that formed a PFCA-HSA complex. Electrostatic interactions were the main intermolecular forces between PFOA and HSA, while hydrogen bonding and van der Waals interactions played important roles in the combination of PFDA and HSA. In fact, the binding of PFDA to HSA was stronger than that of PFOA as supported by fluorescence quenching and molecular docking. In addition, infrared spectroscopy demonstrated that the binding of PFOA/PFDA resulted in a sharp decrease in the β-sheet and α-helix conformations of HSA. Our results indicated that the carbon chain length of PFCAs had a great impact on its binding affinity, and that PFCAs with longer carbon chains bound more strongly.
Keywords
Carbon chain length; Fluorescence; Human serum albumin (HSA); Molecular docking; Perfluoroalkyl carboxylic acids (PFCAs); Toxicity
Tags
PFAS
•
Additional PFAS (formerly XAgency)
•
Expanded PFAS SEM (formerly PFAS 430)
Litsearch Update: November 2021
Web of Science
Perfluorooctane
•
PFAS 150
Literature Search Update December 2021
PubMed
WOS
Missing 2021 searches
Literature Search Update December 2020
PubMed
Literature Search August 2019
PubMed
Perfluorohexanoic acid
Perfluorooctane
1,1,1,3,3,3-Hexafluoro-2- (fluoromethoxy)propane
•
PFBA
LitSearch: May 2019 - May 2020
PubMed
WoS
Scopus: April 2021
June 2022 Pelch Database
•
PFDA
Literature Search Update 5/2020
PubMed
Literature Search Update 4/2021
PubMed
WoS
Scopus: April 2021
June 2022 Pelch Database
Submitted to EPA
•
PFHxA
LitSearch Update: May 2019 - May 2020
PubMed
WoS
Scopus: April 2021
Results pulled from Pelch database May 2022
HAWC
•
PFOA (335-67-1) and PFOS (1763-23-1)
LitSearch: Feb 2019 - May 2020
PubMed
WoS
Literature Search Update (Apr 2019 - Sep 2020)
PubMed
WOS
•
PFOA and PFOS OW MCLG Approaches
Cited in White Papers
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