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HERO ID
6326274
Reference Type
Journal Article
Title
Highly sensitive UPLC-MS/MS method for the quantification of paromomycin in human plasma
Author(s)
Roseboom, IC; Thijssen, B; Rosing, H; Mbui, J; Beijnen, JH; Dorlo, TPC
Year
2020
Is Peer Reviewed?
Yes
Journal
Journal of Pharmaceutical and Biomedical Analysis
ISSN:
0731-7085
EISSN:
1873-264X
Publisher
Elsevier B.V.
Location
AMSTERDAM
Volume
185
Issue
Elsevier
Page Numbers
113245
Language
English
PMID
32199328
DOI
10.1016/j.jpba.2020.113245
Web of Science Id
WOS:000532677100030
URL
https://linkinghub.elsevier.com/retrieve/pii/S0731708519330833
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Abstract
A highly sensitive method was developed to quantitate the antileishmanial agent paromomycin in human plasma, with a lower limit of quantification of 5 ng/mL. Separation was achieved using an isocratic ion-pair ultra-high performance liquid chromatographic (UPLC) method with a minimal concentration of heptafluorobutyric acid, which was coupled through an electrospray ionization interface to a triple quadrupole - linear ion trap mass spectrometer for detection. The method was validated over a linear calibration range of 5 to 1000 ng/mL (r2≥0.997) with inter-assay accuracies and precisions within the internationally accepted criteria. Volumes of 50 μL of human K2EDTA plasma were processed by using a simple protein precipitation method with 40 μL 20 % trichloroacetic acid. A good performance was shown in terms of recovery (100 %), matrix effect (C.V. ≤ 12.0 %) and carry-over (≤17.5 % of the lower limit of quantitation). Paromomycin spiked to human plasma samples was stable for at least 24 h at room temperature, 6 h at 35 °C, and 104 days at -20 °C. Paromomycin adsorbs to glass containers at low concentrations, and therefore acidic conditions were used throughout the assay, in combination with polypropylene tubes and autosampler vials. The assay was successfully applied in a pharmacokinetic study in visceral leishmaniasis patients from Eastern Africa.
Keywords
Paromomycin; Human plasma; Ion-pair liquid chromatography; Tandem mass spectrometry; Assay; Bioanalytical validation
Tags
PFAS
•
Additional PFAS (formerly XAgency)
•
PFAS 150
Missing 2021 searches
•
PFBA
LitSearch: May 2019 - May 2020
PubMed
Scopus: April 2021
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