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HERO ID
673550
Reference Type
Journal Article
Title
Effects of peroxisome proliferators on rat liver phospholipids: sphingomyelin degradation may be involved in hepatotoxic mechanism of perfluorodecanoic acid
Author(s)
Adinehzadeh, M; Reo, NV
Year
1998
Is Peer Reviewed?
Yes
Journal
Chemical Research in Toxicology
ISSN:
0893-228X
EISSN:
1520-5010
Publisher
American Chemical Society
Volume
11
Issue
5
Page Numbers
428-440
Language
English
PMID
9585473
DOI
10.1021/tx970155t
Web of Science Id
WOS:000073747000005
URL
https://search.proquest.com/docview/79868314?accountid=171501
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Abstract
Perfluorooctanoic acid (PFOA), perfluorodecanoic acid (PFDA), clofibrate, di(2-ethylhexyl)phthalate (DEHP), and Wy-14,643 represent a class of compounds known as peroxisome proliferators (PPs). Such compounds induce biogenesis of liver peroxisomes and cause a varying degree of hepatotoxicity and carcinogenesis in rodents. We examined the effects of these PPs on rat hepatic lipids and phospholipid profiles using phosphorus-31 NMR spectroscopy. All PPs caused a 25-57% increase in hepatic phospholipid content, while all but clofibrate increased the total lipid content by 26-156%. Treatments also influenced the composition of liver phospholipids. Phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEth) contents were significantly increased in all treatment groups. Most notably, PFDA caused the largest increase in PtdCho and PtdEth content (ca. 70%), while PFOA and Wy-14,643 were the only test compounds that influenced the PtdCho:PtdEth ratio. PFDA also caused an ca. 30% decrease in sphingomyelin (SphM) from 24 to 120 h postdose. SphM is a key lipid in signal transduction processes involved in apoptosis. Hydrolysis of SphM can be mediated through the action of tumor necrosis factor (TNF-alpha). We measured the TNF-alpha concentrations in rat sera at 24 h post-PFDA-exposure and found an 8-fold increase relative to vehicle-treated controls. These data demonstrate that an increase in the serum TNF-alpha level correlates with the time frame for the observed reduction in hepatic SphM. PFOA, a structurally similar compound, had no effect on hepatic SphM content, nor did it affect the serum TNF-alpha concentration. These effects may be related to differences in the tumorigenicity associated with these compounds. We postulate that PFDA activates the SphM signal transduction pathway via the release of TNF-alpha. This then stimulates cytotoxic responses and processes of apoptosis and may suppress cell proliferative and mitogenic responses.
Keywords
Decanoic Acids; Fluorocarbons; Phospholipids; Sphingomyelins; Tumor Necrosis Factor-alpha; perfluorodecanoic acid; 335-76-2; Index Medicus; Tumor Necrosis Factor-alpha -- metabolism; Animals; Magnetic Resonance Spectroscopy; Rats, Inbred F344; Lipids -- chemistry; Lipid Metabolism; Microbodies -- drug effects; Liver -- metabolism; Decanoic Acids -- toxicity; Liver -- drug effects; Fluorocarbons -- toxicity; Sphingomyelins -- metabolism; Chemical and Drug Induced Liver Injury -- metabolism; Phospholipids -- metabolism
Tags
PFAS
•
Additional PFAS (formerly XAgency)
Literature Search November 2019
Other Sources
TEDX
Screened Studies
Excluded
Exclude (TIAB)
•
Expanded PFAS SEM (formerly PFAS 430)
Litsearch: September 2019
Other Sources
PFAS TOX Database
Screened Studies
Excluded
Exclude (TIAB)
•
^Per- and Polyfluoroalkyl Substances (PFAS)
PFDA (335-76-2)
Literature Search
Pubmed
WOS
Toxline
•
PFAS 150
Literature Search August 2019
PubMed
Web of Science
ToxNet
Other sources
PFAS TOX Database
Not prioritized for screening
Ammonium perfluorooctanoate
Perfluorodecanoic acid
Perfluorooctanoic acid
•
PFDA
Literature Search
Pubmed
Toxline
WOS
Scopus: April 2021
Screening Results
In vivo animal studies
Liver tox
Title and Abstract Screening
Full Text Screening
Tagged as Supplemental
Mechanistic or MOA
non-PECO route of exposure
June 2022 Pelch Database
Submitted to EPA
Literature Searches (through April 2023 update and post-public comment/peer review)a
Other
Published PFAS SEMs
Title & Abstract Screening
Tagged as Supplemental
Full Text Screening
In vivo MOA, including non-PECO exposure route
•
Yale PFAS Liver study
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