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Citation
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HERO ID
697307
Reference Type
Journal Article
Title
IgE, COX-2, and IL-4 are Expressed by DEHP through p38 MAPK and Suppressed by Plant Glycoprotein (75 kDa) in ICR Mice
Author(s)
Oh, P; Lim, K
Year
2011
Is Peer Reviewed?
Yes
Journal
Inflammation
ISSN:
0360-3997
EISSN:
1573-2576
Publisher
SPRINGER/PLENUM PUBLISHERS
Location
NEW YORK
Volume
34
Issue
5
Page Numbers
326-34. [Inflammation]
Language
English
PMID
20686831
DOI
10.1007/s10753-010-9238-8
Web of Science Id
WOS:000294820200004
URL
http://www.springerlink.com/index/10.1007/s10753-010-9238-8
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Abstract
The aim of the present study was to evaluate the inhibitory effect of glycoprotein isolated from Cudrania tricuspidata Bureau (CTB glycoprotein) on di(2-ethylhexyl) phthalate (DEHP)-induced allergic inflammatory response in mice. We evaluated the activity of beta-hexosaminidase, expression of cyclooxygenase (COX)-2, p38 mitogen-activated protein kinase (MAPK), and activator protein (AP)-1, and production of immunoglobulin (Ig)E and interleukin (IL)-4 in DEHP-treated RBL-2H3 cells and ICR mice. Our results revealed that the CTB glycoprotein inhibited the activity of beta-hexosaminidase and production of IgE and IL-4 in serum from DEHP-treated mice. We also found that the CTB glycoprotein reduced arachidonic acid release, COX-2 expression, and AP-1 transcriptional activation through p38 MAPK phosphorylation in DEHP-treated RBL-2H3 cells. The activation of AP-1 was completely blocked by treatment with p38 MAPK inhibitor (SKF86002). The results from these experiments indicate that CTB glycoprotein effectively protects against the allergic inflammation response, mainly through downregulation of MAPK/AP-1 in the mast cell degranulation stage. In conclusion, we suggest that the CTB glycoprotein may be one component of health supplements for the prevention of allergic inflammation.
Keywords
Cudrania tricuspidata Bureau (CTB) glycoprotein; di(2-ethylhexyl) phthalate; immunoglobulin E; interleukin-4; arachidonic acid
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Phthalates – Targeted Search for Epidemiological Studies
Excluded
Source - August 2017 Update (Private)
WOS - Forward Search Results
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