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HERO ID
700543
Reference Type
Journal Article
Title
Glutamine transaminase K is not a major cysteine S-conjugate beta-lyase of rat kidney mitochondria: Evidence that a high-molecular weight enzyme fulfills this role
Author(s)
Abraham, D; Thomas, R; Cooper, A
Year
1995
Is Peer Reviewed?
1
Journal
Molecular Pharmacology
ISSN:
0026-895X
EISSN:
1521-0111
Volume
48
Issue
5
Page Numbers
855-860
Language
English
PMID
7476916
Web of Science Id
WOS:A1995TG28800010
URL
http://molpharm.aspetjournals.org/content/48/5/855.full
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Abstract
Glutamine transaminase K (homodimer; M(r) of monomer approximately 45,000) is a major cysteine S-conjugate beta-lyase of rat kidney cytosol. Several cysteine S-conjugates are known to cause kidney damage. Mitochondria are especially sensitive, and glutamine transaminase K activity is present in the mitochondrial fraction of rat kidneys. Therefore, it is possible that the mitochondrial form of glutamine transaminase K is a cysteine S-conjugate beta-lyase of the rat kidney and that this activity contributes to the mitochondrial damage. However, the literature contains conflicting data on this point. We obtained highly purified mitochondrial glutamine transaminase K and showed that it possesses little cysteine S-conjugate beta-lysae activity with S-(1,2-dichlorovinyl)-L-cysteine and S-(1,1,2,2-tetrafluoroethyl)-L-cysteine as substrates. Recently, a high-molecular-weight cysteine S-conjugate beta-lyase (M(r) approximately 330,000) was shown to be present in the cytosol of rat kidney homogenates and partially purified. We present evidence that suggests that a similar high-molecular-weight enzyme is present in rat kidney mitochondria and that this protein may be a major cysteine S-conjugate beta-lyase of these organelles.
Tags
IRIS
•
Trichloroethylene (TCE) (Final, 2011)
All References
Toxicokinetics
Metabolism
OPPT REs
•
OPPT_Trichloroethylene (TCE)_C. Engineering
Total – title/abstract screening
Off topic
•
OPPT_Trichloroethylene (TCE)_D. Exposure
Total – title/abstract screening
Off topic
•
OPPT_Trichloroethylene (TCE)_E. Fate
Total – title/abstract screening
Off topic
•
OPPT_Trichloroethylene (TCE)_F. Human Health
Total – title/abstract screening
Off topic
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