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Citation
Tags
HERO ID
784902
Reference Type
Journal Article
Title
CALUX measurements: Statistical inferences for the dose-response curve
Author(s)
Elskens, M; Baston, DS; Stumpf, C; Haedrich, J; Keupers, I; Croes, K; Denison, MS; Baeyens, W; Goeyens, L
Year
2011
Is Peer Reviewed?
1
Journal
Talanta
ISSN:
0039-9140
EISSN:
1873-3573
Volume
85
Issue
4
Page Numbers
1966-1973
Language
English
PMID
21872045
DOI
10.1016/j.talanta.2011.07.014
Abstract
Chemical Activated LUciferase gene eXpression [CALUX] is a reporter gene mammalian cell bioassay used for detection and semi-quantitative analyses of dioxin-like compounds. CALUX dose-response curves for 2,3,7,8-tetrachlorodibenzo-p-dioxin [TCDD] are typically smooth and sigmoidal when the dose is portrayed on a logarithmic scale. Non-linear regression models are used to calibrate the CALUX response versus TCDD standards and to convert the sample response into Bioanalytical EQuivalents (BEQs). Several complications may arise in terms of statistical inference, specifically and most important is the uncertainty assessment of the predicted BEQ. This paper presents the use of linear calibration functions based on Box-Cox transformations to overcome the issue of uncertainty assessment. Main issues being addressed are (i) confidence and prediction intervals for the CALUX response, (ii) confidence and prediction intervals for the predicted BEQ-value, and (iii) detection/estimation capabilities for the sigmoid and linearized models. Statistical comparisons between different calculation methods involving inverse prediction, effective concentration ratios (ECR(20-50-80)) and slope ratio were achieved with example datasets in order to provide guidance for optimizing BEQ determinations and expand assay performance with the recombinant mouse hepatoma CALUX cell line H1L6.1c3.
Tag
IRIS
•
Dioxin (2012 Project Page for Final Report)
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