Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
787528
Reference Type
Journal Article
Title
Effect of lead and cadmium co-exposure on testicular steroid metabolism and antioxidant system of adult male rats
Author(s)
Pandya, C; Pillai, P; Nampoothiri, LP; Bhatt, N; Gupta, S; Gupta, S
Year
2012
Is Peer Reviewed?
Yes
Journal
Andrologia
ISSN:
0303-4569
EISSN:
1439-0272
Volume
44
Issue
S1
Page Numbers
813-822
Language
English
PMID
21933223
DOI
10.1111/j.1439-0272.2010.01137.x
Web of Science Id
WOS:000304439300118
Abstract
The mechanism of testicular toxicity of lead (Pb) and cadmium (Cd) is poorly understood. Previous studies focused on single metal-related changes in testicular toxicity. This study points towards the possible involvement of Pb- and Cd-induced oxidative stress in the suppression of steroidogenesis. The oxidative status of testis of adult male rats exposed to Pb acetate and cadmium acetate either alone or in combination at a dose of 0.025 mg kg(-1) body weight of metal intraperitoneally for 15 days was studied. Pb and Cd caused an increase in reactive oxygen species (ROS) by elevating testicular malondialdehydes (MDA) and decrease in activities of testicular antioxidant enzymes superoxide dismutase (SOD), catalase, glucose 6 phosphate dehydrogenase (G6PDH) and glutathione-S-transferase (GST) in mitochondrial and/or post-mitochondrial fraction. Activities of steroidogenic enzymes 3β and 17β-hydroxysteroid dehydrogenase also decreased significantly leading to altered testosterone production. Metal-exposed groups showed significantly decreased testicular and epididymal sperm count. Epididymal sperm motility and viability was also decreased on Pb and Cd exposure. Cd exposure showed more toxic effect than lead exposure, while combined exposure demonstrated least toxicity. In vitro experiments showed that vitamin C restores steroidogenic enzyme activities, suggesting that Pb- and Cd-induced ROS inhibits the testicular steroidogenesis.
Keywords
Antioxidant enzyme; cadmium; lead; reactive oxygen species; testis
Tags
NAAQS
•
ISA-Lead (2013 Final Project Page)
Considered
Cited
1st Draft
2nd Draft
3rd Draft
Final
Health Effects
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity