Determination of [14C]leucine specific radioactivity utilizing high-performance liquid chromatography to isolate the double-labeled dinitrophenyl methyl ester | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

8350896

Reference Type

Journal Article

Title

Determination of [14C]leucine specific radioactivity utilizing high-performance liquid chromatography to isolate the double-labeled dinitrophenyl methyl ester

Author(s)

Bump, EA; Reed, DJ

Year

1977

Is Peer Reviewed?

Yes

Journal

Analytical Biochemistry
ISSN: 0003-2697
EISSN: 1096-0309

Volume

Issue

Page Numbers

527-534

Language

English

DOI

10.1016/0003-2697(77)90113-0

Abstract

A rapid, accurate, and sensitive method for the determination of leucine specific radioactivity was developed. It is based upon a popular technique in which a second label is introduced by reaction with radioactive fluorodinitrobenzene, allowing a determination of specific radioactivity from the 14C3H ratio in the isolated derivative. The present method utilized high-performance liquid chromatography to isolate the methyl ester of the dinitrophenyl derivative. Resolution of the methyl-DNP-leucine from the corresponding derivatives of all other amino acids was achieved on small particle silica packings in an elution time of 6 min. Application to the determination of specific radioactivity of other amino acids is possible. © 1977.