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HERO ID
990502
Reference Type
Journal Article
Title
Effect of primary degradation-reaction products from Ammonia Fiber Expansion (AFEX)-treated corn stover on the growth and fermentation of Escherichia coli KO11
Author(s)
Lau, MW; Dale, BE
Year
2010
Is Peer Reviewed?
Yes
Journal
Bioresource Technology
ISSN:
0960-8524
EISSN:
1873-2976
Volume
101
Issue
20
Page Numbers
7849-7855
Language
English
PMID
20627718
DOI
10.1016/j.biortech.2010.04.076
Abstract
The primary degradation-reaction products (DRP) identified in Ammonia Fiber Expansion (AFEX)-pretreated corn stover are acetate, lactate, 4-hydroxybenzaldehyde (4HBD) and acetamide. The effects of these products at a broad concentration range were tested on Escherichia coli KO11, a strain engineered for cellulosic ethanol production. Fermentations using glucose or xylose as the sole carbohydrate source and a sugar mixture of glucose and xylose were conducted to determine how these products and sugar selection affected fermentation performance. Co-fermentation of the sugar mixture exhibited the lowest overall ethanol productivity compared to single-sugar fermentations and was more susceptible to inhibition. Metabolic ethanol yield increased with the increasing initial concentration of acetate. Although these degradation-reaction products (with exception of acetamide) are generally perceived to be inhibitory, organic acids and 4-hydroxybenzaldehyde at low levels stimulated fermentation. Adaptation of cells to these products prior to fermentation increased overall fermentation rate.
Tags
IRIS
•
Ammonia
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