Abstract  Lots of chemicals are produced in chemical industry and used everywhere as convenient and indispensable materials in daily life and industry. Moreover, many new chemicals are needed to produce competitive new goods such as new medicines, new dyestuffs, new agricultural chemicals and others. Main chemicals used in industry have reached to more than 50,000 kinds. And many workers are exposed to chemicals and injured all over the world. To protect the workers in small workplaces against hazardous chemicals is one of the most important tasks of occupational health. n-Hexane, lead and 1-bromopropane poisoning are shown as examples for health hazards and preventive measures in small workplaces. Preventive measure such as TLV or OEL, Material Safety Data sheets, health check-up, comprehensive cooperation among employers, workers, researchers, industrial physicians and administrative officers, and information on toxicity are discussed.

Abstract  We reported recently that 1-bromopropane (1-BP; n-propylbromide, CAS Registry no. 106-94-5), an alternative to ozone-depleting solvents, is neurotoxic and exhibits reproductive toxicity in rats. The four most recent case reports suggested possible neurotoxicity of 1-BP in workers. The aim of the present study was to establish the neurologic effects of 1-BP in workers and examine the relationship with exposure levels. We surveyed 27 female workers in a 1-BP production factory and compared 23 of them with 23 age-matched workers in a beer factory as controls. The workers were interviewed and examined by neurologic, electrophysiologic, hematologic, biochemical, neurobehavioral, and postural sway tests. 1-BP exposure levels were estimated with passive samplers. Tests with a tuning fork showed diminished vibration sensation of the foot in 15 workers exposed to 1-BP but in none of the controls. 1-BP factory workers showed significantly longer distal latency in the tibial nerve than did the controls but no significant changes in motor nerve conduction velocity. Workers also displayed lower values in sensory nerve conduction velocity in the sural nerve, backward recalled digits, Benton visual memory test scores, pursuit aiming test scores, and five items of the Profile of Mood States (POMS) test (tension, depression, anxiety, fatigue, and confusion) compared with controls matched for age and education. Workers hired after May 1999, who were exposed to 1-BP only (workers hired before 1999 could have also been exposed to 2-BP), showed similar changes in vibration sense, distal latency, Benton test scores, and depression and fatigue in the POMS test. Time-weighted average exposure levels in the workers were 0.34-49.19 ppm. Exposure to 1-BP could adversely affect peripheral nerves or/and the central nervous system.

Abstract  Epidemiologic studies can measure exposure to volatile organic compounds (VOCs) using environmental samples, biomarkers, questionnaires, or observations. These different exposure assessment approaches each have advantages and disadvantages; thus, evaluating relationships is an important consideration. In the National Children's Vanguard Study from 2009 to 2010, participants completed questionnaires and data collectors observed VOC exposure sources and collected urine samples from 488 third trimester pregnant women at in-person study visits. From urine, we simultaneously quantified 28 VOC metabolites of exposure to acrolein, acrylamide, acrylonitrile, benzene, 1-bromopropane, 1,3-butadiene, carbon disulfide, crotonaldehyde, cyanide, N,N-dimethylformamide, ethylbenzene, ethylene oxide, propylene oxide, styrene, tetrachloroethylene, toluene, trichloroethylene, vinyl chloride, and xylene exposures using ultra high performance liquid chromatography coupled with an electrospray ionization tandem mass spectrometry (UPLC-ESI/MSMS) method. Urinary thiocyanate was measured using an ion chromatography coupled with an electrospray ionization tandem mass spectrometry method (IC-ESI/MSMS). We modeled the relationship between urinary VOC metabolite concentrations and sources of VOC exposure. Sources of exposure were assessed by participant report via questionnaire (use of air fresheners, aerosols, paint or varnish, organic solvents, and passive/active smoking) and by observations by a trained data collector (presence of scented products in homes). We found several significant (p < 0.01) relationships between the urinary metabolites of VOCs and sources of VOC exposure. Smoking was positively associated with metabolites of the tobacco constituents acrolein, acrylamide, acrylonitrile, 1,3-butadiene, crotonaldehyde, cyanide, ethylene oxide, N,N-dimethylformamide, propylene oxide, styrene, and xylene. Study location was negatively associated with the toluene metabolite N-acetyl-S-(benzyl)-l-cysteine (BMA), and paint use was positively associated with the xylene metabolites 2-methylhippuric acid (2MHA) and 3-Methylhippuric acid & 4-methylhippuric acid (3MHA + 4MHA). A near-significant (p = 0.06) relationship was observed between acrylamide metabolites and observation of incense.

Abstract  OBJECTIVE: To examine (1) common types of organic solvent work and prevalent types of solvent used, and (2) possible association of high solvent concentration with types of solvent work and with enterprise size.

METHODS: The present survey was conducted in Kyoto, Japan, in April 2004 to March 2005. Air samples were collected in 1,010 solvent workplaces (SWPs) (>or=5 samples/SWP) in 156 enterprises of various sizes, and analyzed for 47 legally designated organic solvents by flame-ionization detector-equipped gas-liquid chromatography. The geometric mean value of the concentrations (after summation by use of the additiveness formula) in the >or=5 samples were taken as a representative value for the SWP. Solvent works were classified into 11 categories according to the Japanese regulation. Enterprises were classified in terms of number of employees.

RESULTS: Degreasing (including cleaning and wiping) was the most common type of solvent work, followed by painting and printing, and toluene was the most often detected solvent (i.e., in 42% of the 1,010 SWPs). Further observation by types of solvent work disclosed that toluene was most common in printing (61%), painting (78%), and adhesive spreading/adhesion SWPs (47%), whereas isopropyl alcohol was the leading solvent in cases of surface coating (51%) and degreasing/cleaning/wiping SWPs (42%). Use of methyl alcohol was also high (36% of all cases). In contrast, use of hexane in adhesives was limited (12%). There was a reverse size-dependency in solvent concentrations in air of SWPs, being five times higher in enterprises with <or=50 employees as compared with the level in enterprises with >or=501 employees. Among SWPs, concentrations tended to be high in printing workplaces especially in small enterprises. In contrast, the levels were much lower in testing and research laboratories irrespective of enterprise size. Comparison with the results in a previous survey in 1996 (Ukai et al. 1997) showed that use of toluene and xylenes was reduced and use of isopropyl alcohol and methyl alcohol was increased. The need of continuous updating of the target analyte list was stressed in relation to the limitation of the present study.

CONCLUSIONS: Solvent levels were about five times higher in small enterprises as compared with the levels in large enterprises. There was a gradual shift in solvent use from aromatics to other solvents, typically alcohols. The use of hexane in adhesives was reduced. Solvent levels were relatively high in solvent-drying and printing workplaces and low in degreasing/cleaning/wiping workplaces and testing/research laboratories.

Abstract  Objective: This study was mainly focused on styudy on he proteome profile change between exposure to 1-Bromopropane (1-BP) and 1-BP poisoning. Methods: The samples of serums from exposure to 1-BP and 1-BP poisoning were collected and analyzed through Label free proteome technology platform. The differently expressed proteins between the two groups were quantified and identified, followed by function analysis by bioinformatics. Results: 127 proteins over 2 fold-change were selected, in which 39 proteins were up-regulated and 88 proteins were down-regulated. These different-ly expressed proteins were mainly involved in the process of enzyme active regulation, inflammatory reaction, protein modification, stress response, coagulation, transport. Conclusion: The differently expressed proteins provided the potential protein biomarkers for the early diagnosis of 1-BP poisoning and was beneficial for clinical diagnosis of 1-BP and understanding of the mechanism of 1-BP poisoning.

Abstract  10 household products, eight waxes or polishes, and two detergents have been screened by a headspace technique for the emission of volatile organic compounds (VOC). Using Tenax sampling and analysis by combined gas chromatography-mass spectrometry (GC-MS) more than 80 single compounds or groups of isomers have been identified, and semiquantitative data on the initial emission rates (emission rate immediately after application) have been obtained. Products fall into two groups: those containing water as a main (80 to 90%) constituent and those without water. The initial emission rate of the first group ranged from 0.2 to 2 μg cm−2min−1, whereas the second group had higher emission rates of up to 430 μg cm−2min−1. Products of the second group emit essentially hydrocarbons (alkanes, alkenes, and terpenes), whereas most products of the first group emit oxygenated compounds including terpene alcohols and their acetates, aliphatic alcohols and esters, and alkoxy-alcohols.