Haemoglobin adduct formation by acrylonitrile in rats and mice
Fennell, TR; Macneela, JP; Turner, MJ; Swenberg, JA
| HERO ID | 1008333 |
|---|---|
| Year | 1991 |
| Title | Haemoglobin adduct formation by acrylonitrile in rats and mice |
| Book Title | Human carcinogen exposure: Biomonitoring and risk assessment |
| Authors | Fennell, TR; Macneela, JP; Turner, MJ; Swenberg, JA |
| Editor | Garner, RC; Farmer, PB; Steel, GT; Wright, AS |
| Publisher Text | IRL Press at Oxford University Press |
| City | Oxford, England |
| Volume | Biomonitoring and Risk Assessment |
| Page Numbers | 241-246 |
| Abstract | A study was conducted on adducts formed between hemoglobin and acrylonitrile (107131) (AN). Male Fischer-344-rats and male B6C3F1-mice were gavaged with up to 28mg/kg or 10mg/kg radiolabeled AN, respectively, and blood was collected either 24 or 72 hours later. The formation of hemoglobin/acrylonitrile adducts was assessed by chromatography. The reaction of erythrocytes from rats or mice and labeled acrylonitrile was also studied in-vitro, and the extent of globin binding was measured by scintillation counting following globin isolation. Rat hemoglobin bound a greater amount of acrylonitrile compared with mice; however, the shape of the dose response curves was similar in both species. A nonlinear binding curve was seen with higher levels of binding at higher doses. The major peaks detected from hydrolyzed rat and mice globin samples and in-vitro treated samples had the same retention times as S-(2-carboxyethyl)-cysteine. The formation of this species was postulated to result from the direct reaction of AN with cysteine in hemoglobin yielding S-(2-cyanoethyl)cysteine, which was detected as S-(2-carboxyethyl)cysteine following acid hydrolysis. |
| Is Certified Translation | No |
| Dupe Override | No |
| Isbn | 9780199631858 |
| Comments | Journal: Human Carcinogen Exposure ISSN: |
| Is Public | Yes |
| Keyword | <?xml version="1.0" encoding="UTF-8"?><kw>DCN-230017</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Nitriles</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Chemical reactions</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Chemical kinetics</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Metabolism</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Comparative toxicology</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Hemoproteins</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Dose response</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Biological monitoring</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Chromatographic analysis</kw> |
| Is Qa | No |
| Relationship(s) |