Simultaneous extraction and analysis by high performance liquid chromatography coupled to diode array and mass spectrometric detectors of bixin and phenolic compounds from annatto seeds
Chisté, RC; Yamashita, F; Gozzo, FC; Mercadante, AZ
HERO ID
1054677
Reference Type
Journal Article
Year
2011
Language
English
PMID
| HERO ID | 1054677 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Simultaneous extraction and analysis by high performance liquid chromatography coupled to diode array and mass spectrometric detectors of bixin and phenolic compounds from annatto seeds |
| Authors | Chisté, RC; Yamashita, F; Gozzo, FC; Mercadante, AZ |
| Journal | Journal of Chromatography A |
| Volume | 1218 |
| Issue | 1 |
| Page Numbers | 57-63 |
| Abstract | This study was designed to identify and quantify the carotenoids and phenolic compounds from annatto seeds using high performance liquid chromatography coupled to diode array and mass spectrometer detectors (HPLC-DAD-MS/MS). Furthermore, using response surface methodology, an optimized procedure for simultaneous extraction of these compounds was established. In addition to bixin, known to be the main carotenoid in annatto seeds, hypolaetin and a caffeoyl acid derivative were identified as the main phenolic compounds. The optimized procedure involved 15 extractions using acetone:methanol:water (50:40:10, v/v/v) as solvent, a solid-liquid ratio of 1:9 (m/v) and an extraction time of 5 min. Validation data indicated that the HPLC method proposed provided good linearity, sensitivity, procedure accuracy, system precision and suggested its suitability for the simultaneous analysis of phenolic compounds and carotenoids in annatto seeds. |
| Doi | 10.1016/j.chroma.2010.10.094 |
| Pmid | 21111424 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000286494100007 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Bixa orellana L.; Carotenoids; Phenolic compounds; Response surface methodology; HPLC-DAD-MS/MS |
| Is Qa | No |