Characterization of capsaicin-sensitive afferent nerve-mediated gastroprotective action against ammonia-induced gastric mucosal lesion

Shibata, M; Yamaura, T; Onodera, S; Inaba, N; Chida, Y; Ohnishi, H; Murakami, M

HERO ID

1061027

Reference Type

Journal Article

Subtype

Abstract

Year

1992

Language

English

HERO ID 1061027
Material Type Abstract
In Press No
Year 1992
Title Characterization of capsaicin-sensitive afferent nerve-mediated gastroprotective action against ammonia-induced gastric mucosal lesion
Authors Shibata, M; Yamaura, T; Onodera, S; Inaba, N; Chida, Y; Ohnishi, H; Murakami, M
Journal Gastroenterology
Volume 102
Issue 4 pt 2
Page Numbers A163
Abstract FRG-8813 is a histamine H,-antagonist with potent antisecretory and gastroprotective effects. The aim of this study was to examine the property of its protective effect against ammonia-induced mucosal lesion. Methods; (1)Male S.D. rats (180-200g) fasted overnight were treated orally with FRG-8813 30 min before the administration of 1% ammonia. Gastric lesion (mm') was counted 1hr later. In time course study, rats were killed 5, 30 and 60 min after the ammonia treatment, respectively. (2)In order to measure transmucosal potential difference (PD), the stomach of anesthetized rat was mounted on a Lucite chamber. FRG-8813 (0. 1-3 mg/kg) was applied into the chamber for 30 min. The stomach was exposed for another 10 min to 0.25% ammonia after the removal of FRG-8813. After the removal of ammonia, the perfusion systew was resumed. Results; (1)FRG-8813 produced a dose dependent gastroprotective action. The EDs 0 value (60 min) was 3.3 mg/kg p.o. The data of the time course study were as follows; the protective effect was not observed 5 min later. (the brackets contain information in a table) [damaged area (llllll2) by a NH, 5 min 30 min 60 min Control 36.1 ± 9.2 33.0 ±10.2 36.7 ± 6.0 FRG-8813 10mg/kg p.o. 37.1 ± 8.0 12.2 ± 2.8 13.4 ± 4.3• Inhibition (%) -3.8 63.0 63.5 mean± S.E. '; p<0.01 vs Control N=9] (2)The application of FRG-8813 failed to prevent the abrupt PD reduction by 0.25% ammonia at any doses, but it significantly promoted the recovery of the PD reduction dose-dependently. Conclusion: These results suggest that FRG-8813 showed potent gastroprotective effect of the ammonia lesion and the protective effect of FRG-8813 may be attributable to the acceleration of mucosal regeneration after damage.
Is Certified Translation No
Dupe Override No
Conference Location San Francisco, CA
Conference Name 93rd Annual Meeting Of The Anierican Gastroenterological Association
Conference Date May 9-15, 1992
Is Public Yes
Language Text English
Keyword CONGRESSES; BIOLOGY; BIOCHEMISTRY; ANATOMY, COMPARATIVE; HISTOLOGY, COMPARATIVE; REGENERATION; TRANSPLANTATION; DIGESTIVE SYSTEM DISEASES/PATHOLOGY; DIGESTIVE SYSTEM/PATHOLOGY; NERVOUS SYSTEM/PHYSIOLOGY; NERVOUS SYSTEM/METABOLISM; POISONING; ANIMALS, LABORATORY; MURIDAE; General Biology-Symposia; Biochemical Studies-General; Anatomy and Histology; Digestive System-Pathology; Nervous System-Physiology and Biochemistry; Toxicology-General; 7664-41-7
Is Qa No