The influence of storage time and artificial sweat on the ercutaneous absorption of explosives from soils
Reifenrath, W; Reddy, G; Major, M; Leach, G
HERO ID
1065802
Reference Type
Journal Article
Subtype
Abstract
Year
2003
Language
English
| HERO ID | 1065802 |
|---|---|
| Material Type | Abstract |
| In Press | No |
| Year | 2003 |
| Title | The influence of storage time and artificial sweat on the ercutaneous absorption of explosives from soils |
| Authors | Reifenrath, W; Reddy, G; Major, M; Leach, G |
| Journal | Toxicologist |
| Volume | 72 |
| Issue | S-1 |
| Page Numbers | 381 |
| Abstract | We determined the influence of sample storage time on the percutaneous absorption of C-14 labeled hexahydro-1, 3, 5-trinitro-1, 3, 5-triazine (RDX), 2, 6-dinitrotoluene (26DNT) and 2, 4, 6-trinitrotoluene (TNT) from two' soil types, Yolo having 1.9% carbon and Tinker having 9.5% carbon content. RDX soil samples stored at -20C for 27 months and 62 months were compared to freshly spiked soil samples. Similarly, 26DNT samples stored 35-36 months and TNT samples stored 18 months were compared to freshly spiked samples. Approximately 10 ug/cm2 of radio labeled compound was applied in 10 mg/ cm2 of soil to freshly excised pig skin pretreated with artifical sweat (5 ul) and mounted in skin penetration-evaporation chambers, Radiolabel recovered from the dermis and tissue culture media (receptor fluid) was summed to determine percent absorption from the soils. For each compound, percent absorptions of label were highest from Yolo soil. Storage did not significantly alter percutaneous absorption values for RDX, as values were all less than 1%, regardless of soil type or age. Similarly, 26DNT absorption was 1-2% for Tinker soil and 16-18% for Yolo soil, regardless of soil age. TNT absorption was approximately 0.5% from Tinker soil and 3-4% from Yolo soil for fresh and stored samples. HPLC analysis of26DNT in receptor fluid at maximum flux indicated no metabolism or breakdown. For TNT, extensive conversion to monoarnino derivaties and other metabolites was observed. The absorption of 26DNT from low carbon soil was reduced from 16-J 8% to near zero without sweat pretreatment, indicating that skin surface moisture was a critical variable in determining topical bioavailability. |
| Is Certified Translation | No |
| Dupe Override | No |
| Conference Location | Salt Lake City, UT |
| Conference Name | 42nd Annual Meeting of the Society of Toxicology |
| Conference Date | March 9-13, 2003 |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |