Extraction and purification of Ipomoea peroxidase employing three-phase partitioning
Narayan, AV; Madhusudhan, MC; Raghavarao, KS
HERO ID
1071037
Reference Type
Journal Article
Year
2008
Language
English
PMID
| HERO ID | 1071037 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | Extraction and purification of Ipomoea peroxidase employing three-phase partitioning |
| Authors | Narayan, AV; Madhusudhan, MC; Raghavarao, KS |
| Journal | Applied Biochemistry and Biotechnology |
| Volume | 151 |
| Issue | 2-3 |
| Page Numbers | 263-272 |
| Abstract | Three-phase partitioning (TPP) is a novel separation process used for the extraction and purification of biomolecules. The biomolecules are recovered in a purified form at the interface (precipitate), while the contaminants partition in t-butanol and aqueous phases. Peroxidase from the leaves of Ipomoea palmata was purified by using TPP. The ratio of the crude extract to t-butanol of 1:1 and 30% ammonium sulfate at 37 degrees C resulted in about 160% activity recovery and twofold purification in the aqueous phase of the first cycle of TPP. On subjecting the aqueous phase to the second cycle of TPP, a purification of 18-fold was achieved with about 81% activity recovery. The sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis showed substantial purification, and the molecular weight of peroxidase was found to be 20.1 KDa. The present study shows a higher degree of purification and activity yield as a primary purification process in comparison with existing literature values, thus demonstrating TPP as an attractive downstream process for the purification of peroxidase. |
| Doi | 10.1007/s12010-008-8185-4 |
| Pmid | 18369532 |
| Wosid | WOS:000260538600016 |
| Url | https://www.proquest.com/scholarly-journals/extraction-purification-ipomoea-peroxidase/docview/48174081/se-2?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Ammonium Sulfate; Chemical Fractionation; Electrophoresis, Polyacrylamide Gel; Ipomoea; Isoelectric Point; Peroxidase; Plant Leaves; Temperature; tert-Butyl Alcohol; Ipomoea palmata; purification; extraction; chemical constituents of plants; enzyme activity; polyacrylamide gel electrophoresis; butanol; article |
| Is Qa | No |