Biodegradation of ether pollutants by Pseudonocardia sp. strain ENV478
Vainberg, S; Mcclay, K; Masuda, H; Root, D; Condee, C; Zylstra, GJ; Steffan, RJ
HERO ID
1071132
Reference Type
Journal Article
Year
2006
Language
English
PMID
| HERO ID | 1071132 |
|---|---|
| In Press | No |
| Year | 2006 |
| Title | Biodegradation of ether pollutants by Pseudonocardia sp. strain ENV478 |
| Authors | Vainberg, S; Mcclay, K; Masuda, H; Root, D; Condee, C; Zylstra, GJ; Steffan, RJ |
| Journal | Applied and Environmental Microbiology |
| Volume | 72 |
| Issue | 8 |
| Page Numbers | 5218-5224 |
| Abstract | A bacterium designated Pseudonocardia sp. strain ENV478 was isolated by enrichment culturing on tetrahydrofuran (THF) and was screened to determine its ability to degrade a range of ether pollutants. After growth on THF, strain ENV478 degraded THF (63 mg/h/g total suspended solids [TSS]), 1,4-dioxane (21 mg/h/g TSS), 1,3-dioxolane (19 mg/h/g TSS), bis-2-chloroethylether (BCEE) (12 mg/h/g TSS), and methyl tert-butyl ether (MTBE) (9.1 mg/h/g TSS). Although the highest rates of 1,4-dioxane degradation occurred after growth on THF, strain ENV478 also degraded 1,4-dioxane after growth on sucrose, lactate, yeast extract, 2-propanol, and propane, indicating that there was some level of constitutive degradative activity. The BCEE degradation rates were about threefold higher after growth on propane (32 mg/h/g TSS) than after growth on THF, and MTBE degradation resulted in accumulation of tert-butyl alcohol. Degradation of 1,4-dioxane resulted in accumulation of 2-hydroxyethoxyacetic acid (2HEAA). Despite its inability to grow on 1,4-dioxane, strain ENV478 degraded this compound for > 80 days in aquifer microcosms. Our results suggest that the inability of strain ENV478 and possibly other THF-degrading bacteria to grow on 1,4-dioxane is related to their inability to efficiently metabolize the 1,4-dioxane degradation product 2HEAA but that strain ENV478 may nonetheless be useful as a biocatalyst for remediating 1,4-dioxane-contaminated aquifers. |
| Doi | 10.1128/AEM.00160-06 |
| Pmid | 16885268 |
| Wosid | WOS:000239780400011 |
| Url | https://www.proquest.com/scholarly-journals/biodegradation-ether-pollutants-pseudonocardia-sp/docview/68713159/se-2 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: ISSN: |
| Is Public | Yes |
| Language Text | English |
| Keyword | Pseudonocardia; biodegradation; pollutants; organochlorine compounds; bioremediation; aquifers; groundwater contamination; ribosomal RNA; nucleotide sequences |
| Is Qa | No |