Enzymatic esterification of beta-methylglucoside with acrylic/methacrylic acid in organic solvents

Park, DW; Haam, S; Ahn, IS; Lee, TG; Kim, HS; Kim, WS

HERO ID

1071301

Reference Type

Journal Article

Year

2004

Language

English

PMID

14711498

HERO ID 1071301
In Press No
Year 2004
Title Enzymatic esterification of beta-methylglucoside with acrylic/methacrylic acid in organic solvents
Authors Park, DW; Haam, S; Ahn, IS; Lee, TG; Kim, HS; Kim, WS
Journal Journal of Biotechnology
Volume 107
Issue 2
Page Numbers 151-160
Abstract The enzymatic esterifications of beta-methylglucoside with acrylic acid/methacrylic acid were carried out using Novozym 435. t-Butanol indicating the highest conversion value was determined as an optimal solvent. The molar ratio (beta-methylglucoside:acids) of 1:15 was most favorable to the esterification. The enzyme concentration of 5% (w/v), and the temperature (50 degrees C for beta-methylglucoside:acrylic acid, 45 degrees C for beta-methylglucoside:methacrylic acid) resulted in the highest final conversion. Beta-methylglucoside of 60gl(-1) was found to be most effective in terms of short reaction time as well as product concentrations. Under these conditions, the maximum conversions for the esterification of beta-methylglucoside with acrylic acid and beta-methylglucoside with methacrylic acid were 59.3% after 12h and 71.3% after 72h, respectively. The structural analysis of the products was performed by FT-IR spectroscopy and (1)H NMR.
Pmid 14711498
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Dupe Override No
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Language Text English
Is Qa No