Role of CD28-B7 interactions in generation and maintenance of CD8 T cell memory
Suresh, M; Whitmire, JK; Harrington, LE; Larsen, CP; Pearson, TC; Altman, JD; Ahmed, R
| HERO ID | 1103816 |
|---|---|
| In Press | No |
| Year | 2001 |
| Title | Role of CD28-B7 interactions in generation and maintenance of CD8 T cell memory |
| Authors | Suresh, M; Whitmire, JK; Harrington, LE; Larsen, CP; Pearson, TC; Altman, JD; Ahmed, R |
| Journal | Journal of Immunology |
| Volume | 167 |
| Issue | 10 |
| Page Numbers | 5565-5573 |
| Abstract | Although the role of CD28-B7 interaction in the activation of naive T cells is well established, its importance in the generation and maintenance of T cell memory is not well understood. In this study, we examined the requirement for CD28-B7 interactions in primary T cell activation and immune memory. Ag-specific CD8 T cell responses were compared between wild-type (+/+) and CD28-deficient (CD28(-/-)) mice following an acute infection with lymphocytic choriomeningitis virus (LCMV). During the primary response, there was a substantial activation and expansion of LCMV-specific CD8 T cells in both +/+ and CD28(-/-) mice. However, the magnitude of the primary CD8 T cell response to both dominant and subdominant LCMV CTL epitopes was approximately 2- to 3-fold lower in CD28(-/-) mice compared with +/+ mice; the lack of CD28-mediated costimulation did not lead to preferential suppression of CD8 T cell responses to the weaker subdominant epitopes. As seen in CD28(-/-) mice, blockade of B7-mediated costimulation by CTLA4-Ig treatment of +/+ mice also resulted in a 2-fold reduction in the anti-LCMV CD8 T cell responses. Loss of CD28/B7 interactions did not significantly affect the generation and maintenance of CD8 T cell memory; the magnitude of CD8 T cell memory was approximately 2-fold lower in CD28(-/-) mice as compared with +/+ mice. Further, in CD28(-/-) mice, LCMV-specific memory CD8 T cells showed normal homeostatic proliferation in vivo and also conferred protective immunity. Therefore, CD28 signaling is not necessary for the proliferative renewal and maintenance of memory CD8 T cells. |
| Pmid | 11698427 |
| Wosid | WOS:000172106400010 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |