Detection of human lung cytochromes P450 that are immunochemically related to cytochrome P450IIE1 and cytochrome P-450IIIA
Wheeler, CW; Wrighton, SA; Guenthner, TM
HERO ID
11085
Reference Type
Journal Article
Year
1992
Language
English
PMID
| HERO ID | 11085 |
|---|---|
| In Press | No |
| Year | 1992 |
| Title | Detection of human lung cytochromes P450 that are immunochemically related to cytochrome P450IIE1 and cytochrome P-450IIIA |
| Authors | Wheeler, CW; Wrighton, SA; Guenthner, TM |
| Journal | Biochemical Pharmacology |
| Volume | 44 |
| Issue | 1 |
| Page Numbers | 183-187 |
| Abstract | We have used monoclonal antibodies that were prepared against and specifically recognize human hepatic cytochromes P450 as probes for solid phase radioimmunoassay and Western immunoblotting to directly demonstrate the presence in human lung microsomes of cytochromes P450 immunochemically related to human liver cytochromes P450IIE1 (CYP2E1) and P450IIIA (CYP3A).The detected levels of these cytochromes are much lower than levels in human liver microsomes, but similar to the levels seen in microsomes from untreated baboon lung. Proteins immunochemically related to two other constitutive hepatic cytochromes P450, cytochrome P450IIC8 (CYP2C8) andcytochrome P450IIC9 (CYP2C9), were not detectable in lung microsomes. |
| Doi | 10.1016/0006-2952(92)90055-N |
| Pmid | 1632834 |
| Wosid | WOS:A1992JF16000026 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | ECRIB.Biochem. Pharmacol. 44: 183-186. |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |