Preparative isolation and purification of four prenylflavanones from microbial biotransformation of kurarinone by high-speed counter-current chromatography
Ma, XC; Sun, CK; Huang, SS; Wang, JK; Zhang, BJ; Li, FY; Wang, G; Deng, S; Cui, JA
| HERO ID | 1116409 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | Preparative isolation and purification of four prenylflavanones from microbial biotransformation of kurarinone by high-speed counter-current chromatography |
| Authors | Ma, XC; Sun, CK; Huang, SS; Wang, JK; Zhang, BJ; Li, FY; Wang, G; Deng, S; Cui, JA |
| Journal | Separation and Purification Technology |
| Volume | 76 |
| Issue | 2 |
| Page Numbers | 140-145 |
| Abstract | a- A rapid high-speed counter-current chromatography (HSCCC) method was developed successfully for isolation four transformed products in one-step. a- The novel chemical structures were identified as 4a, 5a-dihydroxy norkurarinone, 7-methoxyl-4a, 5a-dihydroxy norkurarinone and 6a-hydroxykurarinone on the basis of the extensive spectral methods. A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of four prenylflavanones from the crud extract after microbial biotransformation of kurarinone was developed successfully using a stepwise elution with two-phase solvent system composed n-hexane-ethyl acetate-methanol-water at the volume ratios of 1:1:0.7:1 (v/v) and 1:1:1.2:1 (v/v). A total of 7mg of 4a,5a-dihydroxy norkurarinone (1), 11mg of 7-methoxyl-4a,5a-dihydroxy norkurarinone (2), 14mg of 6a-hydroxykurarinone (3) and 10mg of norkurarinone (4) were obtained in one-step separation from 520mg of the crude biotransformation sample with purities of 94.0%, 97.8%, 99.5% and 98.7%, respectively. Among them, compounds 1-3 are novel compounds, and their chemical structures were identified on the basis of the extensive spectral methods such as UV, IR, HR-MS and NMR. |
| Doi | 10.1016/j.seppur.2010.09.033 |
| Wosid | WOS:000286089800007 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000286089800007Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-78649448028&doi=10.1016%2fj.seppur.2010.09.033&partnerID=40&md5=23a3651521f24b16e7392764fb5b1b5e |
| Is Public | Yes |
| Language Text | English |
| Keyword | Kurarinone; Biotransformation; Counter-current chromatography; Separation |
| Is Qa | No |